Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis

Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis

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摘要 Either triethylamine or diethylenetriamine can be conveniently used as a dynamic modifier to suppress the adsorption of basic proteins in capillary zone electrophoresis. Sufficiently high column efficiencies (> 2x10(5) plates/m for cytochrome C) were obtained with either of them along with the improvement of the peak shapes and repeatability of migration time. The relationship between the adsorption suppression effect of the modifier and its structure was discussed. Either triethylamine or diethylenetriamine can be conveniently used as a dynamic modifier to suppress the adsorption of basic proteins in capillary zone electrophoresis. Sufficiently high column efficiencies (> 2x10(5) plates/m for cytochrome C) were obtained with either of them along with the improvement of the peak shapes and repeatability of migration time. The relationship between the adsorption suppression effect of the modifier and its structure was discussed.

作者 Ying Hua QIU Al Jin HUANG Yi Liang SUN(Department of Chemistry. Peking University. Beijing 100871)

出处《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第3期227-230,共4页 中国化学快报（英文版）

关键词 capillary zone electrophoresis basic proteins dynamic modifier triethylamne DIETHYLENETRIAMINE capillary zone electrophoresis basic proteins dynamic modifier triethylamne diethylenetriamine

分类号 O623.732 [理学—有机化学]

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Chinese Chemical Letters

1999年第3期

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Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis

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