摘要
生物处理技术,是从自然界中选育菌株来处理废水的原始形式,即利用废水中的有毒污染物作为唯一碳源,微生物利用其进行生长,从而达到使废水降解的效果。以草甘膦废水为唯一碳源,通过选择性富集培养法、驯化培养法、划线分离和纯化,从湖北宜昌某化工集团污水处理厂排污口活性污泥中分离得到菌株XF150。三次驯化过程中,菌株XF150降解草甘膦废水最高降解率达95.52%。经观察形态特征及培养特征,初步鉴定菌株XF150为青霉菌属(Penicillium sp).。由单因子优化法实验法探讨了温度、pH值、底物浓度对菌株XF150降解草甘膦废水的影响,得出菌株XF150降解草甘膦废水的最适条件:温度为30℃、pH为7.0、底物浓度为600mL/L,在最适条件下菌株对草甘膦废水降解率可达84.18%,其矿化程度较高,为草甘膦废水的生物处理技术提供最佳处理方案。
Biological treatment technologies as original form of wastewater treatment by breeding strains from nature achieve the effect of wastewater degradation by using toxic pollutants in wastewater as sole carbon source and microorganisms for its growth.Strain XF150 utilizing glyphosate wastewater as only carbon was isolated from an outfall of the wastewater treatment plant of a chemical works in Yichang through selective enrichment,acclimation training,crossed separation and purification.At three acclimation training,degradation rate of glyhphosate wastewater reached up to 95.52%.It was preliminary identified as Penicillium sp through straining and cultural characteristics.Effects of temperature,pH,and concentration of wastewater on biodegradation were discussed.Under the optimum conditions of strain XF150 degradation 30℃ ,pH 7.0,wastewater concentration 600mL/L by the single factor experiment,it was found that degradation rate of glyphosate wastewater reached up to 84.18% ,with the highest mineralization degree,to provide the best treatment program for glyphosate biological wastewater treatment technologies.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2010年第8期28-32,共5页
Environmental Science & Technology
基金
湖北省优秀中青年团队研究项目(T200703)
湖北省青年杰出人才基金(2005ABB030)
湖北省教育厅自然科学重大项目资助(2004z001)
关键词
草甘膦废水
生物降解
活性污泥
glyphosate wastewater
biodegradation
activated sludge