摘要
分别以抗、感纹枯病的玉米自交系CML429、DM9为材料,研究接种纹枯病菌后喷施水杨酸对玉米叶片过氧化物酶、超氧化物歧化酶活性及产量的影响。结果表明,接种纹枯病菌后5d内,两玉米自交系叶片SOD活性先下降后升高并回到初始水平,而POD活性则表现持续的升高,CML429的SOD、POD活性均明显高于DM9。用不同浓度的水杨酸处理玉米叶片后,两个玉米自交系的SOD活性均逐渐下降,而POD则先略有下降后逐渐升高,在达到高峰后大部分处理有所回落。此外,所有外源水杨酸处理均能增强高感玉米自交系DM9对纹枯病的抗性,明显提高玉米产量,平均产量比对照高17.97%,而对抗性自交系的产量没有明显效果,以0.5mmol/L的处理效果最好。因此,外源水杨酸处理能降低玉米植株体内的活性氧水平,减轻纹枯病菌的伤害。
In order to investigate the effects of exogenous salicylic acid （SA） on the activities of peroxidase （POD）, superoxide dismutase （SOD） in leaf and yield of two maize lines with different resistance to sheath blight, CML429 and DM9 were inoculated with Rhizoctonia solani （R. solani） and then sprayed with SA under field conditions. The results showed that the SOD activity in leaf of two maize lines decreased at first and then increased to the initial level, while the POD activity showed a gradual increase in five days after inoculation, and the activities of SOD and POD for CML429 were higher than that of DM9. After spraying different concentrations of SA to leaf of maize, the SOD activity in leaf of two maize lines de- creased gradually, while the POD activity decreased at first, and then increased gradually to the peak and declined slightly at later stage. Moreover, the exogenous SA could promote the resistance to sheath blight and the yield of high susceptible lines DM9 obviously, the average yield was 13.7% higher than that of the control, while there was no obvious effects on CML429. Among all SA treatments, 0.5 mmol/L SA had the best effect under field conditions. Therefore, exogenous SA could decrease the active oxygen in plant of maize to reduce the damage of sheath blight.
出处
《广西农业科学》
CSCD
2009年第3期242-245,共4页
Guangxi Agricultural Sciences
基金
广西农科院科技发展基金项目(2004029)
关键词
玉米
纹枯病
水杨酸
立枯丝核菌
超氧化物歧化酶
过氧化物酶
maize （Zea Mays L）
sheath blight
salicylic acid
Rhizoctonia solani
superoxide dismutase
peroxidase
