摘要
目的 探讨整合素-Ⅱ2基因（integrin alpha-2，ITGA2）C807T和整合素-β3基因（integrin beta-3，ITGB3）T176C多态性与缺血性脑卒中的关系及其对血脂、脂蛋白水平的影响。方法 应用聚合酶链反应-限制性片段长度多态性和DNA测序的方法检测265例缺血性脑卒中患者和280名对照组ITGA2和ITGB3的基因型；同时按常规方法测定血浆脂质、脂蛋白水平。结果 缺血性脑卒中组总胆固醇（total cholesterol，TC）、甘油三酯（triacylglycerol，TG）、低密度脂蛋白-胆固醇（low density lipoproteincholesterol，LDL-C）水平明显高于对照组（P〈0．05），ITGB3基因T176C多态性在缺血性脑卒中组和正常人群中的分布差异无统计学意义（P〉0．05），而ITGA2基因C807T多态性在两组人群中的分布差异有统计学意义（P〈0．05），等位基因频率的相对风险分析发现，T等位基因携带者患缺血性脑卒中的风险是C等位基因的1．455倍（OR=1．455，95％CI：1．134～1．866），携带T等位基因的缺血性脑卒中个体血浆TC水平显著高于不携带者（P〈0．05）。结论 ITGA2基因C807T多态性与缺血性脑卒中的发病具有相关性，其中T等位基因可能是缺血性脑卒中的遗传易感基因；ITGA2基因C807T多态性可能通过影响血脂水平而影响缺血性脑卒中的发生。
Objective To study the association of integrin alpha-2 （ITGA2） gene C807T, integrin beta-3（ITGB3 ） gene T176C polymorphisms with ischemic stroke and the effect of the polymorphisms on plasma lipid and lipoprotein levels. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and DNA sequencing were used to detect the integrin genotypes in 265 patients with ischemic stroke and 280 healthy controls. The plasma lipid and lipoprotein levels were measured by routine method. Results Plasma total cholesterol （TC）, triacylglycerol （TG） and low density lipoproteincholesterol （LDL-C） in the patients with ischemic stroke were significantly higher than those in the controls （P〈0. 05）. The distributions of the ITGB3 gene T176C polymorphism were not different between the ischemic stroke group and control group, but the ITGA2 gene C807T polymorphism was significantly different. The relative risk suffering from ischemic stroke of the T allele carrier was 1. 455 times as that of the C allele carrier （OR= 1. 455,95%CI：1. 134-1. 866）. The level of plasma lipid in the T allele carriers was significantly higher than that in the C allele carriers （P〈0. 05）. Conclusion The ITGA2 gene C807T polymorphism was associated with ischemic stroke, the 807 T allele may be a genetic risk factor for ischemic stroke. The ITGA2 gene C807T polymorphism may affect ischemic stroke through plasma lipid and lipoprotein levels.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2009年第2期211-215,共5页
Chinese Journal of Medical Genetics
基金
国家自然科学基金（30660159）
