摘要
目的通过RNA干扰(RNAi)阻断宫颈癌细胞HeLa229中核因子(NF)-κB信号通路,观察其单独或与顺铂(DDP)联合对HeLa229增殖、耐药性和侵袭力的影响。方法利用RNAi技术,将HeLa229分为未转染组(A组)和转染p65小干扰RNA(siRNA)组(B组),用MTT法检测转染p65 siRNA 24、48、72 h时HeLa229的增殖情况;加入不同浓度DDP,观察其对HeLa229细胞增殖的影响。用Boyden chamber体外侵袭实验检测A、B组及p65siRNA与DDP(8.6 mg/L)联合组(C组)细胞侵袭力的变化。结果B组细胞存活率较A组明显下降;加入DDP后,A、B组细胞的存活率均随DDP浓度的增加而下降。siRNA与DDP联合应用可明显提高HeLa229对DDP的敏感性。与A组相比,B、C组穿越Matrigel胶的细胞数明显减少(P<0.05)。结论应用RNAi技术可有效阻断HeLa229内NF-κB信号通路,抑制其增殖和体外侵袭力,增强其对DDP的敏感性。
Objective To investigate cell proliferation and invasiveness of cervical cancer HeLa229 cell after inhibition of the NF-κB signaling pathway by p65 siRNA or in combination with cisplatin. Methods The HeLa229 cell was divided into untransfected group （A group） and transfected p65 siRNA group （B group）. Cell viability was detected by MTT after HeLa229 cell were transfected with or without p65 siRNA for 24, 48, 72 h. The sensitivity to DDP of the HeLa229 ceil, transfected with or without p65 siRNA, was evaluated also by MTT. Boyden chamber experiment in vitro was used to detect the invasion ability of two groups and p65 siRNA ＋ DDP group （ C group）. Results p65 siRNA inhibited the ceil proliferation compared with the untransfected cells. Proliferations of both cells transfected with and without p65 siRNA were inhibited in a concentration-dependent manner, while at the same concentration of DDP the viability of B group cell was significantly suppressed （ P 〈 0.05）. Compared with the A group cells, the number of B and C group ceils traversed Matrigel were decreased obviously （P 〈 0.05）. Conclusion RNAi targeting of p65 has anti-proliferative effects, inhibits the invasiveness and increases the DDP sensitivity of the HeLa229 cells.
出处
《山东医药》
CAS
北大核心
2008年第36期26-27,共2页
Shandong Medical Journal
基金
教育部"十五"211工程重点建设项目(2002-2)。
