摘要
目的体外观察正常及损伤状态下的心肌细胞(CMs)培养上清液诱导人骨髓间充质干细胞(hMSCs)向心肌样细胞分化的作用。方法体外分离培养hMSCs及乳鼠原代CMs,制备正常搏动状态及损伤状态下的CMs培养上清液,分别对传代hMSCs培养诱导27～30d。倒置显微镜观察两组的细胞形态,免疫细胞化学染色检测两组心肌细胞特异性标志物cTnI及Desmin的表达。结果hMSCs经正常搏动CMs上清诱导培养后,只有少数细胞变宽大,部分细胞表达cTnI,但不表达Desmin;经损伤CMs上清诱导培养后,hMSCs变为宽大,大部分细胞表达cTnI,部分细胞表达Desmin。定量分析,正常与损伤状态下培养上清液诱导细胞表达cTnI、desmin的阳性百分比及阳性表达强度OD值均有非常显著性差异(P<0.01)。结论正常与损伤状态下CMs培养上清液均可诱导hMSCs向心肌样细胞分化,CMs损伤状态下的细胞培养上清液更有利于促进其分化。
Objective To observe whether human bone marrow mesenchymal stem cells （hMSCs） could differentiate into cardio- myo-like cells by culturing with supernatant of normal or injured rat cardiomyocytes （CMs） in vitro. Methods hMSCs were cultured with supernatant from normal or injured rat CMs for 27-30 d. The morphologies of induced hMSCs were observed with inverted microscope and the special cardio-markers cTnI and Desmin were identified with immunocytochemisry. Results A few cells cultured with supernatant from normal CMs enlarged and expressed cTnI, but little Desmin. While more cells cultured with supernatant from injured CMs enlarged and expressed cTnI, and parts of them expressed Desmin. The incidence of cTnI or Desmin positive cells were significantly different between these two groups （P〈0.01）. Conclusion Supernatant from both normal and injured CMs can induce hMSCs into cardio-like cells in vitro, and that from injured CMs is more effectively.
出处
《中国康复理论与实践》
CSCD
2009年第3期228-230,I0001,共4页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家高技术研究发展计划863计划(2004AA205020)
