摘要
目的用Touchdown PCR检测野生中缅树鼩的伯氏疏螺旋体感染。方法从昆明及其周边地区捕获30只野生中缅树鼩,乙醚深度麻醉后处死,取内脏-30℃保存备用。用总DNA提取试剂盒提取组织总DNA,用紫外可见光分光光度计测定样品的DNA含量,用热启动Touchdown PCR技术检测野生中缅树鼩的伯氏疏螺旋体flaB基因。对PCR产物做凝胶电泳,凝胶成像仪观察并照相。结果Touchdown PCR有良好的特异性和敏感性。实验重复3次,结果一致,野生树鼩的伯氏疏螺旋体DNA阳性率为63.33%(19/30)。结论野生树鼩存在伯氏疏螺旋体感染,并且感染率较高。
Objective To investigate the status of Borrelia burgdorferi infection in wild tree shrews （Tupaia belangeri） in Yunnan Province. Methods There 30 wild tree shrews were captured in Kunming and its suburban areas. Animals were anesthetized with overdosed ethoxyethane, 3-oxapentane and sacrificed by euthanasia, their internal organs were collected and frozen in icebox at-30oC before use. Total DNA samples were extracted from tissues and determined by UV spectrometer. Touchdown PCR were performed to detect tab gene of Borrelia burgdorferi in DNA samples. Agarose gel electrophoresis was used to isolate PCR products, and Gel imagine system was used to check PCR products and take gel photos. Results Touchdown PCR was highly specific and sensitive. Borrelia burgdorferi infection rate was 63.33%, （19/30） in wild tree shrew population in Yunnan. Conclusion There is Borrelia burgdorferi infection in wild tree shrew population in Yunnan and the infection rate is high.
出处
《中国热带医学》
CAS
2009年第4期621-622,734,共3页
China Tropical Medicine
基金
云南省科技计划项目平台项目(No.2006PT07-1)
