摘要
样品中的孔雀石绿、结晶紫经试剂盒提取,浓缩后用经固相萃取柱净化、硼氢化钾还原、反向色谱柱分离,使用荧光检测器检测,孔雀石绿、结晶紫的加标回收率在76.1%～92.5%之间,相对标准偏差1.9%～5.8%,检出限均为0.5μg/kg。结果表明:该方法检测孔雀石绿(MG)、结晶紫(GV)处理简单,灵敏度高,节省时间,可用于大量样品的快速分析。
Malachite green and gentian violet residues in fishery products were determined by high performance liquid chromatography method coupled with fluorescence detector. Samples were extracted by pre-treatment kit for extraction of MG, LMG, GV, LGV and purified by solid-phase extraction cartridges. After concentrated, samples were deoxidized by potassium borohydride. The MG and GV were detected with FLD detector after C18 column separation. The recoveries for fortified fish tissue were 76.1%～92.5 %, The relative standard deviation（RSD） values for repeatability were 1.9 % - 5.8 %. The detection limit for MG and CV was 0.5μg/kg, respectively.
出处
《广东海洋大学学报》
CAS
2009年第1期54-57,共4页
Journal of Guangdong Ocean University
基金
广东省重大科技兴海项目(B200701A06)
