摘要
目的研究(NF-κB)和(MCP-1)在糖尿病肾病(DN)大鼠肾组织中的变化并探讨黄芪对NF-κB和MCP-1表达的影响及其对糖尿病大鼠肾脏的保护作用。方法将大鼠随机分成正常对照组(C组)、糖尿病组(DM组)、糖尿病黄芪组(DT组);生化法测定血肌酐及24h尿蛋白;免疫组织化学法检测各组大鼠肾组织NF-κB、MCP-1的表达。结果C组肾小球NF-κB及MCP-1在有少量表达,DT组肾小球中NF-κB、MCP-1表达较DM组有下降(P<0.05);DM组血肌酐和24h尿蛋白较C组升高(P<0.01),而DT组较DM组明显降低(P<0.05);DT组较DM组病理学损害有改善。结论黄芪能减少尿蛋白、延缓肾功能进展、改善肾组织病理学损害,对糖尿病肾病大鼠有治疗作用,其机制之一可能是通过抑制NF-κB的活化和MCP-1表达而实现的。
Objective To investigate the changes of nuclear factor kappaB （NF-κB） and Monocyto Chemoatractant Protein-1 （MCP-1） in renal tissues of diabetic rat and the protective function of astragalus in diabetic rats. Methods SD male rats were randomly divided into three groups which were normal control group （C）, diabetic group （DM）, and astragalus treated group （DT）. The expressions of NF-κB and MCP-1 were measured by Immuno-histochemstry. The levels of creatinine （Cr） and 24- hour urine protein excretion were measured by Biochemistry. Results Immunohistochemstry revealed that the expression of NF-κB and MCP-1 in glomeruli significantly increased in DM group （P〈0. 01） and the expression of them in DT group more decreased than that in DM group （P〈0. 05）. The levels of 24-hour urine protein and Cr in DM group were higher than those in DT group （P〈0. 05）. Compared with DM group, examination of kidney sections in DT group rats showed that renal histology changes including mesangium expansion, proliferation of mesangial cells, infiltration of mononuclear cells and extraeellular matrix accumulation were markedly improved. Conclusion These results suggest that the activated NF-κB and expression of MCP-1 plays an pathogenic role in the development of diabetic nephropathy. Astragalus can reduce urine protein excretion, improve renal function, ameliorated renal histology changes and halt progression of diabetic nephropathy. The mechanism perhaps was related to the reduction NF-κB and MCP-1 expression in renal tissues in diabetic rats.
出处
《贵州医药》
CAS
2009年第2期102-105,共4页
Guizhou Medical Journal
