摘要
为了探讨急性冷应激对仔猪免疫系统的影响，本试验将18头45d“约克夏”仔猪随机分成常温对照组（Control，C）、冷暴露组（Coldexposure，CE）和冷暴露并阻断糖皮质激素受体组（Cold exposure＋RU486，CER）．常温组在18-20℃、冷暴露各组在4～8℃环境下分栏饲养．冷暴露时间持续12h，在冷暴露开始后的第0、1、3、6、9、12h六个时间点对18头仔猪采血并分离血浆，分别使用双抗体夹心ELISA法检测各血浆样品的IL－2和IL－6水平，放射免疫分析法测定血浆样品的ACTH和皮质醇水平．结果显示：单纯冷暴露组IL－2水平和IL－6水平在冷暴露6h时明显升高（P〈0．01），ACTH水平在各时间点均明显降低（P〈0．01），而Cortisol水平仅在冷暴露3h时明显升高（P〈O．01），IL-2与IL-6的水平呈强相关（r=0．9595），ACTH与IL-2的水平呈中等强度负相关（r=-0．4359）、与IL-6的水平呈弱的负相关，皮质醇与IL-2的水平呈弱的负相关、与IL-6的水平呈中等强度负相关（r=-0．4478）；受体阻断组IL-2在冷暴露6h、9h和12h时均明显升高（P〈0．01），IL-6在冷暴露6h明显升高（P〈0．01），ACTH在冷暴露各时间点均明显降低（P〈0．01），IL-2和IL-6水平呈中等相关（r=0．4161），ACTH与IL-2、IL-6的水平均呈强负相关（r=-0．6765／-0．7174），皮质醇与IL-2、1L．6均呈弱负相关．上述结果表明，急性冷暴露能使IL-2、IL-6及皮质醇的分泌增强，且阻断糖皮质激素受体会导致IL-2和IL-6之间以及ACTH与IL-2／IL-6之间的相关性改变．
Eighteen Yorkshire piglets aged 45 d were divided into 3 groups randomly. Group 1 （control） was kept at ambient temperature （18-20 ℃）. Group 2 （Cold exposure） was kept at 4-8℃ for 12 hour. Group 3 （Cold exposure ＋ RU486） was disposed by hypodermic injection （20 mg/kg body weight） and kept at temperature of 4-8 ℃ for 12 h. Blood samples were collected at 0 h, 1 h, 3 h, 6 h, 9 h and 12 h after cold exposure from superior vena cava. The levels of IL-2 and 1L-6 in plasma were measured by the method of double antibody sandwich ELISA. The levels of ACTH and cortisol in plasma were evaluated by the RIA （Radio-immunity analysis） method. The results showed that of group 2 the levels of IL-2 and IL-6 increased significantly at 6 h （P〈0.01）, the level of ACTH decreased significantly at 1 h, 3 h, 6 h and 12 h （P〈0.01） and the level of cortisol increased obviously at 3 h （P〈0.01）. There was a strong correlation between the levels of IL-2 and IL-6 （r=0.959 5）, a moderately negative correlation between ACTH and IL-2 （r=-0.435 9）, a weakly negative correlation between ACTH and IL-6, a weakly negative correlation between cortisol and IL-2, and a moderately negative correlation between cortisol and IL-6 （r=-0.447 8）. Of group 3 the 1L-2 level increased remarkably at 6 h, 9 h and 12 h under cold exposure （P〈0.01） and rose slightly at 3 h （P〈0.05）, the level oflL-6 rose obviously at 6 h （P〈0.01） and slightly at I h （P〈0.05）, the level of ACTH decreased dramatically at 1 h, 3 h, 6 h, 9 h and 12 h （P〈0.01）. There was a moderate correlation between the levels of IL-2 and IL-6 （r=0.416 1）, a strongly negative correlation between ACTH and IL-2/IL-6 （r=-0.676 5/-0.717 4） and a weakly negative correlation between cortisol and IL-2/ IL-6. Those results suggested that the acute exposure at low temperature enhanced the secretion of IL-2, IL-6 and cortisol, and the blockage of GR could induce the changes of correlation between IL-2 and IL-6, and ACTH and IL-2/IL-6. Fig 1, Ref21
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2009年第1期91-94,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金(No.30671534)资助项目~~
