摘要
目的了解血管内皮细胞生长因子（ＶＥＧＦ）ｍＲＮＡ在肝细胞癌（ＨＣＣ）和癌周组织中的表达，探讨ＶＥＧＦ与ＨＣＣ侵袭和转移的关系，为在分子水平干预肿瘤血管形成，预防ＨＣＣ复发和转移打下基础。方法采用逆转录—聚合酶链反应（ＲＴ－ＰＣＲ）检测方法，对４３例ＨＣＣ手术标本的癌和癌周组织中ＶＥＧＦｍＲＮＡ的表达水平进行了相对定量研究。结果肿瘤组织中ＶＥＧＦｍＲＮＡ的表达率为７９．１％（３４／４３），癌周组织表达率仅为２０．９％（９／４３）；肿瘤组织中ＶＥＧＦｍＲＮＡ表达水平癌栓组（０．６９７９±０．２３６３）和包膜不完整组（０．４７０２±０．２８８３）分别显著高于无癌栓组（０．３４３６±０．２３９１）和包膜完整组（０．２８７０±０．２５１０，Ｐ＜０．０５）；肿瘤组织中ＶＥＧＦｍＲＮＡ表达水平大肝癌组（０．４０７２±０．２８８４）与小肝癌组（０．３７３４±０．２９００）相比差异无显著性（Ｐ＞０．０５）。结论ＶＥＧＦ在ＨＣＣ浸润和转移过程中发挥重要作用，肿瘤血管形成与ＨＣＣ浸润和转移关系密切。
Objective To study the relationship between the expression of vascular endothelial growth factor (VEGF) mRNA and invasion and metastasis of hepatocellular carcinoma (HCC), and explore new approaches to prevent and inhibit recurrence and metastasis of HCC. Methods Surgical samples of 43 patients with HCC were studied using reverse transcription polymerase chain reaction( RT PCR). The relative level of VEGF mRNA expression was measured by determining a ratio of PCR products of VEGF to that of β actin gene. Results (1) Expression of VEGF mRNA expression in tumor tissue was found in 79.1% (34/43) of patients, whereas it was seen in 20.9%(9/43) of the nontumorous liver tissue. (2) The level of VEGF mRNA expression in tumors with tumor emboli or in non encapsulated tumors was higher than that without tumor emboli or in well encapsulated tumors ( P < 0.05). (3) There was no significant difference in the expression of VEGF mRNA between large HCC (diameter>5 cm) and small HCC (diameter≤5 cm) ( P >0.05). Conclusion VEGF may play an important role in the invasion and metastasis of HCC. Angiogenesis in tumor correlates with the progression of HCC.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1998年第1期12-14,共3页
Chinese Journal of Oncology
基金
上海市医学领先学科基金
美国中华医学基金会基金
关键词
肝肿瘤
肿瘤转移
聚合酶链反应
肝细胞癌
VEGF
Liver neoplasms Carcinoma,hepatocellular Vascular endothelial growth facter Neoplasm metastasis Polymerase chain reaction Neoplasm invasiveness
