摘要
目的体外培养扩增大鼠胚胎神经干细胞（NSCs）及骨髓基质细胞（BMSCs），并移植入大鼠胶质瘤模型体内，观察细胞生物学特性改变及其对胶质瘤的影响。方法取孕15d的大鼠海马区神经干细胞及大鼠股骨骨髓基质细胞，体外培养扩增；建立胶质瘤模型，待肿瘤生长至3周和4周时，分别随机抽取5只大鼠行MRI检测；分不同时间段大鼠灌注取材，分别做苏木素-伊红（HE）染色及BrdU免疫组织化学染色，观察细胞存活情况及对胶质瘤的影响。结果两种细胞移植入胶质瘤模型体内均能够大量存活。BMSCs组、NSCs移植组、对照组模型平均生存时间分别为4．03、4．28、3．88周；3周及4周时肿瘤的平均直径分别为（6．dmm／7．6mm、5．4mm／6．0mm、7．2mm／8．0mm）。BrdU染色示两种细胞均对胶质瘤细胞有定向靶向作用。结论神经干细胞对胶质瘤细胞的生长有抑制作用，能够延长大鼠模型的生存期。骨髓基质细胞对胶质瘤生长无抑制作用。两种细胞均对胶质瘤细胞有定向靶向作用，能够作为生物载体转染目的基因治疗胶质瘤。
Objective After embryo neural stem cells （NSCs） and bone marrow stromal cells （BMSCs） were cultured and amplified in vitro, and transplanted into glioma from rats, to observe the changes of biological characteristics of NSCs and BMSCs and neurospongioma. Methods NSCs and BMSCs were isolated from the hippocampus of the rat embryos （fetal age of 15 days） and cultured in vitro. Establish glioma models and in the 3w and 4w selected 5 rats randomly to enforce MRI detection. Draw the materials after being fixed at the different time, HE staining and BrdU immunohistochemistry. Observe the Cell livability and the impact on glioma. Results NSCs and BMSCs that transplanted into glioma models were able to survive. The average survival time of the BMSCs group, NSCs transplantation group and the control group models respectively is 4.03 weeks, 4.28 weeks, 3.88 weeks ; the average diameter of the glioma when growed three weeks and four weeks respectively is （6.4 mm/7.6 mm,5.4 mm/6.0 mm,7.2 mm/ 8.0 mm）. BrdU staining showed that two types of cells are directed targeting to the glioma cells. Conclusion NSCs can inhibit the growth of glioma cells,extending the survival time of rats. BMSCs have no significant inhibition on the growth of glioma cells. The two types of cells are directed targeting to the glioma cells, and they can be used as biological can＇ier transfering target genes to treat glioma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第3期378-380,共3页
Chinese Journal of Experimental Surgery
基金
基金项目：湖北省科技厅资助项目（2005AA301C28）
湖北省卫生厅2005青年科技人才基金资助项目（QJX2005.15）
郧阳医学院优秀中青年科技创新团队项目（2005CXX02）
关键词
胶质瘤
神经干细胞
骨髓基质细胞
移植
Glioma
Neural stem ceils
Bone marrow stromal cells
Transplant
