摘要
研究了饮水中添加不同水平硼对固始鸡肾脏组织结构的影响。将180羽1日龄固始鸡,随机分为对照组和试验Ⅰ组、Ⅱ组,试验Ⅰ、Ⅱ组饮水中分别含硼100,200mg/L,试验期6周。每周末每组随机选取固始鸡10羽,颈动、静脉放血致死,立即解剖取肾脏,Bouin液固定,制作石蜡切片,H·E染色,显微观察与摄影。结果表明,试验Ⅰ组1～2周肾小体血管球肿胀,肾小管管壁细胞肿胀、变性,集合小管和肾小管直部细胞颗粒变性;第3周起肾脏组织结构开始恢复,到第6周时组织结构基本恢复正常。试验Ⅱ组1～2周固始鸡肾小体血管球的萎缩、坏死、崩解现象较为普遍,肾小囊腔变大,囊壁结构不完整;近曲小管和远曲小管细胞肿胀,细胞颗粒变性或水样变性;3～6周固始鸡肾脏的组织结构有恢复迹象;但第6周肾脏的病理组织学变化仍比较明显。结论:饮水添加硼100mg/L,对1～2周固始鸡肾脏组织结构发育有一定的抑制或损伤作用,对3～6周固始鸡肾脏的组织结构发育有改善和促进作用;饮水添加硼200mg/L,则对整个试验期固始鸡肾脏的组织结构发育均有明显的抑制甚至毒性作用,进而影响机体排泄功能。
The influence of boron with different level added in water on renal organization structure was studied. 180 pcs of 1 d old Gushi chicks were randomly divided into control group, Group Ⅰ and Ⅱ, the drinking water of Group Ⅰ and Ⅱ contained 100 and 200 mg/L boron resp., the experiment period was 6 w. 10 pcs of Gushi chickens were selected at every weekend to slaughter by bloodletting at neck artery and vein, kidney were taken at once in anatomy, fixed with Bouin liquid, paraffin section prepared, H .E staining, microscopic observation and photograph were conducted. The results showed that glomus swelling on renal corpuscle, tubular wall cell swelling of renal tubule, degeneration, denaturalization of collecting tubule and on the straight part cell particles of renal tubule for tested Group Ⅰ at 1-2 w; the renal organization structure began to restore from the 3rd week and organization structure got right at the 6th week. For the Group Ⅱ, at 1-2 w, the phenomena of atrophy, necrosis and disintegration of renal corpuscle glomus for Gushi chickens were common, capsule of glomerulus became bigger, capsule wall structure was not complete, swelling of proximal and distal convoluted tubular cells, denaturalization or hydropic degeneration of cell particles; the renal organization structure of Gushi chickens had recovery sign at 3-6 w; but the renal histopathology change was still evident at the 6th week. Conclusion： There were some inhibitory or damage effects on renal organization structure of Gushi chickens at 1 -2 w by adding 100 mg/L boron in drinking water, and had promotion and improvement effects on renal organization structure growth of chickens at 3 -6 w.It had inhibitory even toxic effect on renal organization structure growth of Gushi chickens by adding 200 mg/L boron in water at the whole test period, so as to affect body excretion function.
出处
《畜牧与饲料科学》
2009年第1期1-4,共4页
Animal Husbandry and Feed Science
基金
安徽省高等学校学科拔尖人才项目(2005043)
安徽省高校青年教师科研资助计划项目(2007jq1106)
关键词
硼
固始鸡
生长性能
肾脏
组织结构
boron
Gushi chickens
growth performance
renal
organization structure
