摘要
目的:研究活血化瘀中药丹参及其组分丹参酚酸B盐(salvianolic acid B,SA-B)对内皮素-1(endothelin-1 ,ET-1)所致门静脉高压(portal hypertension,PHT)模型小鼠肝脏微循环的影响。方法:昆明种小鼠84只,随机分为模型组、内皮素A受体(endothelin A receptor , ETAR)阻断剂组、黄芪组、黄芪多糖组、延胡索组、丹参组、SA-B组,每组12只。预先灌胃等量药物或蒸馏水,1次/d,共3 d。用微量注射泵以80μL/min速度从尾静脉注射ET-1(1 .6μg/kg) ,建立门静脉高压模型。每组中6只小鼠用激光多普勒测定尾静脉注射ET-1前后肝脏平均血流灌注量;另6只活体观察注射ET-1前后肝脏微循环流速。结果:各组注射ET-1后肝脏平均血流灌注量均有不同程度的下降,但SA-B组和ETAR阻断剂组下降幅度较模型组小(P<0 .01) ;黄芪组和黄芪多糖组下降幅度与模型组接近,与SA-B组比较,差异有统计学意义(P<0 .05) ;SA-B组则与ETAR阻断剂组作用相当。从各组肝脏微血管流速的变化来看,SA-B组和ETAR阻断剂组流速下降幅度均小于模型组(P<0 .05 ,P<0 .01) ;丹参组、SA-B组的下降幅度与ETAR阻断剂组相比,差异无统计学意义。结论:丹参和SA-B能对抗ET-1引起的肝脏平均血流灌注量和微循环流速降低,这可能是其改善门静脉高压时肝脏微循环的机制之一。
Objective： To observe the effects of Salviae miltiorrhizae and its component, salvianolic acid B （SA-B）, on the microcirculation of liver in mice with portal hypertension induced by endothelin-1 （ET-1）.
Methods： Eighty-four Kunming mice were randomly divided into 7 groups： untreated group, endothelin A receptor （ETAR） blocker group, Astragali mongolici group, Astragalus polysaccharides （APS） group, Corydalis Yanhusuo group, Salviae miltiorrhizae group and SA-B group. There were 12 mice in each group. Mice were pretreated with a corresponding equivalent volume of drug or distilled water for 3 days, and then the portal hypertension in mice was induced by continuous injection of ET-1 into coccygeal vein using a micro- injection pump. Six mice in each group were used to observe the average liver blood flow volume by laser- Doppler flow instrument before and after injection of ET-1, and the other six rats were used to observe the hepatic microcirculation velocity in vivo by an inverted microscope.
Results： The average blood flow of liver in mice decreased in each group after ET-1 injection. But the changes of average blood flow in the SA-B group and the ETAR blocker group were less than that in the untreated group （P〈0.01）. The changes of average blood volume in the Astragali mongolici group and the APS group were similar to that in the untreated group, but more than that in the SA-B group after injection of ET-1. The change of average blood flow in the SA-B group showed no significant difference when compared with the ETAR blocker group. The microcirculatory flow velocity in liver also decreased in each group after ET-1 injection. But the changes of microcirculatory flow velocity in the SA-B group and the ETAR blocker group were less than that in the untreated group （P〈0. 05, P〈0.01）. There were no significant differences in the changes of microcirculatory flow velocity among the Salviae miltiorrhizae group, the SA-B group and the ETAR blocker group. Conclusion： Salviae miltiorrhizae and SA-B can decrease the average blood flow and microcirculatory flow velocity in liver in mice with portal hypertension, which may be one of the mechanisms of Salviae miltiorrhizae and SA-B in decreasing portal hypertension.
出处
《中西医结合学报》
CAS
2009年第2期151-156,共6页
Journal of Chinese Integrative Medicine
基金
上海市科学技术委员会基金资助项目(No.05DZ19716)
国家自然科学基金资助项目(No.30672761)
关键词
肝硬化
高血压
门静脉
微循环
中药
小鼠
liver cirrhosis
hypertension, portal
microcirculation
drugs, Chinese herbal
mice
