摘要
[目的]在甜高粱丝黑穗病基因的分子标记研究上有所进展。[方法]以甜高粱抗丝黑穗病品种LTR102、甜高粱感丝黑穗病品种LTR106为试材,应用SSR技术对甜高粱丝黑穗病基因进行分子标记的初步分析。试验中采用CTAB方法提取甜高粱基因组。[结果]通过比较Taq酶用量的不同,进一步优化了Taq酶的用量。用SSR分子标记技术对其进行多态性扩增,所用的20个SSR引物中有16个引物能扩增出清晰的多态性谱带,完全可应用于甜高粱丝黑穗病抗病基因的初步定位。其中有4个引物扩增出了差异带,引物号为:Xtxp 279、Xtxp284、Xtxp36、Xtxp228。[结论]通过比较体系中各不同因素的影响,进一步优化了SSR反应体系,达到了较理想的检测效果。
[Objective] The research progress of the molecular marker of sweet sorghum smut wire gene was reviewed.[Method] The sweet sorghum variety with antismut silk——TR102 and the sweet sorghum variety with the sensitive to head smut——LTR106 being used as experimental material,the SSR technology was preliminarily applied in the analysis of sweet sorghum smut silk gene.The sweet sorghum genome can be extracted with the method of CTAB.[Results] The concentration of Taq polymerase was further optimized through the comparison test of the different concentrations of Taq polymerase.The amplified polymorphism of SSR molecular marker was done by 20 SSR primers and 16 primers were able to clearly amplify the polymorphic bands,which can be used in the initial loci of the resistant gene.Among them,the different band was amplified in 4 primers,which were Xtxp 279,Xtxp284,Xtxp36 and Xtxp228,respectively.[Conclusion] The optimization of the SSR reaction system was realized based on the comparison of different factors.
出处
《安徽农业科学》
CAS
北大核心
2008年第36期15794-15796,共3页
Journal of Anhui Agricultural Sciences
基金
辽宁省教育厅重点实验室资助项目(20060806)
