摘要
目的观察肺气虚模型大鼠血内皮素(ET)、白介素-1β(IL-1β)及肿瘤坏死因子-α(TNF-α)含量的变化。方法用气管内注入脂多糖及熏香烟方法复制肺气虚大鼠模型,观察症状、体征以及24h尿量、比重;放免法测定血ET、IL-1β及TNF-α含量;HE染色观察形态学变化。结果模型组大鼠出现咳嗽、气急、鼻部潮红,精神萎靡、蜷伏不动、毛失光泽、食量减少、便稀等症状,同时伴有尿量减少、比重增加;血ET、IL-1β与TNF-α均有升高;肺组织符合慢性支气管炎、肺气肿病理变化。结论血ET、IL-1β及TNF-α含量升高,可作为肺气虚模型大鼠的客观指标。
Objective To observe the change of plasma endothelin（ ET）, IL-1β TNF-α in lungQi deficiency model rats. Methods The rats of lung Qi deficiency were made by injecting lipopolysaccharide into the trachea with cigarette smoking. Behavior,24 h urine volumes and urine specific were observed. The ET,IL-1β and TNF-α concentrations in blood were measured by radioimmunity method and the morphological changes were observed by HE staining. Results The model rats showed coughing, breathlessness, wheezing, humid and minute red in the nasal part, depression, action retardation,immobilility, hair burnishness and shedding, meanwhile with gradually appetite decrease, softening and thin stool. Compared with those of the control group, urine volume decreased markedly and urine density increased obviously in the rats of the model group. Plasma ET, IL-1β and TNF-α concentration in the model group were higher than those of control group rats. The pathological changes of the lung tissue consistented with those of chronic bronchitis and emphysema- Conclusions Concentration increases in blood ET. IL-1β and TNF-α can be the standards of Lung-Qi deficiency model rats.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2008年第24期2414-2416,共3页
Chinese Journal of Gerontology
基金
辽宁省科技厅基金资助项目(No.20062071)
关键词
肺气虚模型
尿量
内皮素
白细胞介素-1Β
肿瘤坏死因子-α
Animal model of lungQi deficiency
Urine volume
Endothelin （ET）
Interleukin-1β （ IL-1β）
Tumor necrosis factor-α（TNF-α）
