摘要
采用红外光谱三级宏观指纹鉴定的方法对刺五加不同部位根、茎、叶原药材及总苷提取物的红外光谱图进行了整体的分析。原药材谱图显示,根和茎谱图较为相似,主要体现草酸钙和淀粉特征峰,而叶的草酸钙特征峰几乎消失,淀粉特征峰的峰形也变得不明显。总苷提取物的一维谱图显示根、茎、叶在1602,1514,1452 cm^-1（苯环骨架振动）和1271 cm^-1（=C-O）附近均有特征吸收,因此,推测三者共有的成分为酚苷类化合物。二阶导数谱图给出叶在1656 cm^-1（黄酮的C=O）附近特征峰明显强于根和茎,说明叶中黄酮类成分高于根和茎。二维谱图在1350-1700 cm^-1波段根和茎均体现苯环骨架振动的5个自动峰1636,1600,1521,1462,1453 cm^-1,叶除了苯环骨架振动峰外还出现1656 cm^-1黄酮类成分的C=O振动,进一步证实叶中黄酮类成分高于根和茎。
In the present paper,raw radix,stem and leaf and the total glycoside extract of them of Acanthopanax senticosus Harms were studied via the multi-steps IR macro-fingerprint method.The spectra of raw medicinal materials show that the spectra of radix and stem are similar and mainly show characteristic peaks of calcium oxalate and starch,whereas the characteristic peaks of calcium oxalate of leaf almost disappear and the shape of characteristic peak of starch also gets unconspicuous.The FTIR spectra of total glycoside extract of radix,stem and leaf present characteristic peaks at 1 602,1 514,1 452 cm^-1（vibration of phenyl framework） and 1 271 cm^-1（=C-O）,respectively,therefore,the authors speculated that their mutual component is the compound of phenolic glycoside.Through observing the second derivative IR spectra of the total glycoside extract of different parts of Acanthopanax senticosus Harms,the authors found that the characteristic peak of leaf is stronger than that of radix and stem at 1 656 cm^-1（flavone C=O）,this proves that the quantity of component of flavone in the leaf is higher than that in the radix and stem.In the two-dimensional correlation spectra,the radix and stem both have five automatic peaks（vibration of phenyl framework） in 1 350-1 700 cm^-1,whereas the leaf still shows another automatic peak at 1 656 cm^-1（flavone C=O）,and this further proves that the quantity of component of flavone in the leaf is higher than that in the radix and stem.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2008年第12期2859-2863,共5页
Spectroscopy and Spectral Analysis
基金
科技部“十五”重大科技攻关项目
科技部重大技术标准研究专项课题项目(2002BA906A29-4)资助
关键词
红外光谱
二阶导数谱
二维相关光谱
刺五加
Infrared spectroscopy
Second derivative IR spectroscopy
Two-dimensional IR correlation spectroscopy
Acanthopanax senticosus Harms
