摘要
用Ｌ２７（３１３）正交试验研究ＸＣＣＮＡＵ－９２生产黄原胶的发酵培养基配方。结果表明，碳源和氮源显著影响黄原胶发酵液的粘度，二者的交互作用对黄原胶产量及发酵液粘度的影响皆显著；黄原胶生产的最佳发酵培养基配方（ｇ／Ｌ）为：玉米淀粉６０．０，氮源Ｘ１．０，ＣａＣＯ３３．０，ＭｇＳＯ４·７Ｈ２Ｏ０．５，Ｋ２ＨＰＯ４·３Ｈ２Ｏ１．０（０．０）。３０℃，１５０ｒｐｍ条件下发酵７２ｈ，工业级黄原胶产量达４０．８４ｇ／ｋｇ，发酵液粘度８６０００ｃｐ，丙酮酸含量４．１％，碳源转化率达６８．１％。
A fermentative medium for XCCNAU-92 producing Xanthan gum was developed by L 27 (3 13 ) orthogonal experiment.The results showed that sources of carbon and nitrogen affected viscosity of the ferment mash significantly and the reciprocity between the two sources all influenced yield of xanthan gum and viscosity of the mash.The optimum medium for xanthan production was put forward as follow:60 0 g/L maize starch,1 0 g/L nitrogenX,3 0 g/L CaCO 3,0 5 g/LMgSO 4·7H 2O,1 0(0 0) g/L K 2HPO 4·3H 2O,After 72 h fermentation under 30℃ (150 rpm) on which industrial grade xanthan gum yield of 40 84 g/kg,viscosity of ferment mash of 86 000 cp,pyruvate concentration of 4 1%,and transformation ratio of carbon of 68 1% could be attained.
出处
《安徽农业大学学报》
CAS
CSCD
1998年第2期191-195,共5页
Journal of Anhui Agricultural University
关键词
黄原胶
正交试验
发酵培养基
配方
Xanthan gum Orthogonal experiment Fermentative medium formula
