摘要
目的观察N-甲基-D-天冬氨酸受体(NMDA)NR2A,NR2B亚基对脑缺血-再灌注后海马CA1区神经细胞存活的不同影响。方法采用Pulsinelli-Brierley四动脉阻塞(4-VO)大鼠全脑缺血模型,缺血前连续3天脑室注射NR2A,NR2B反义寡核苷酸(ASODN)后缺血15min,复灌5d,石蜡切片,以焦油紫染色,图像分析测定单位面积内焦油紫染色细胞面积总和,与缺血组及错义寡核苷酸组(MSODN)进行形态学分析。结果NR2A、NR2B反义寡核苷酸对脑缺血再灌注后海马CA1神经细胞均有明显保护作用,以NR2A AS ODN保护作用更明显,与缺血组比较约有50%细胞存活(P<0.05)。结论NR2A,NR2B亚基的含量降低对脑缺血再灌注后海马神经细胞有明显保护作用。
Objective To investigate the role of NR2A, NR2B [the subunits of N-methyl-Dasparate（NMDA） receptor] on the neuronal death induced by transient cerebral ischemia. Methods Transient （15 rain） brain ischemia was induced by the four-vessel occlusion in Sprague Dawley rats. The antisense oligodeoxynucleotides （ODNs） of NR2A and NR2B were used to suppress the expression of NR2A and NR2B by intracerebroventricular intracerebroventricular infusion once per day for 3 days before ischemia. After 5 days reperfusion, the rats were perfusion -fixed with paraformaldehyde and cresyl violet staining was used to examine the survival of CA1 pyramidal cells of the hippocampus. Results Both NR2A and NR2B antisense ODNs showed a protective effect against neuronal cell death induced by cerebral ischemia/reperfusion. Furthermore, NR2A antisense ODNs had more protective effect on the neuronal death than NR2B antisense ODNs （P〈0. 05）. Conclusion Both NR2A and NR2B suhunits play an important role in neuronal death induced by cerebral ischemia followed by reperfusion.
出处
《江苏医药》
CAS
CSCD
北大核心
2009年第1期81-83,I0004,共4页
Jiangsu Medical Journal
基金
江苏省自然科学基金(BK2006536)
国家自然科学基金(30800309)
