摘要
目的:探究姜黄素抗小鼠B16黑色素瘤生长与Bcl-2、P53、肿瘤细胞内谷胱甘肽的关系。方法:构建C57BL/6 J移植性小鼠B16黑色素瘤模型考察姜黄素对黑色素瘤体生长的影响,MTT法检测姜黄素对B16细胞的增殖活力的影响,流式细胞仪检测细胞凋亡及Bcl-2、P53基因的表达,HPLC分析技术检测姜黄素对C57BL/6 J小鼠B16黑色素瘤组织内GSH水平的影响。结果:姜黄素对C57BL/6 J移植性小鼠B16黑色素瘤生长的抑制作用呈剂量及时间依赖性;姜黄素作用B16细胞24h,Bcl-2蛋白表达减弱,P53蛋白表达增强;姜黄素使C57BL/6 J小鼠B16黑色素瘤组织内的GSH含量下降。结论:姜黄素体内抑制小鼠B16黑色素瘤的生长;体外抑制B16细胞的生长,并能诱导部分细胞凋亡,其作用机制可能与其下调Bcl-2蛋白的表达,上调P53蛋白的表达及耗竭肿瘤组织内GSH含量有关。
Objective：To investigate the effects of curcumine on mouse B16 melanoma growth and possible mechanism of Bcl-2, P53 and glutathione in tumor cells. Methods ： The inhibitory effect on growth of melanoma in vivo were examined by mice melanoma models transplanted B16 cells to C57BL/6J mice. MTT method was used to assay the contribution of eurcumine to B16 cells in vitro. The apoptosis and expression of Bcl-2 ,P53 gene of B16 cells were analyzed by flow eytometry, and HPLC assay was used to detect the change of GSH in B16 melanom tissues of C57BL/6J mouse caused by curcumine. Results： Curcumine had obvious inhibitory effect on the growth of mouse B16 melanom in time and dose dependent manner and the gene expression of bcl-2 in B16 ceils decreased after 24 hours supplied with curcumine, whereas p53 protein expression increased; Curcumine depressed the GSH quantity in melanoma tissues. Conclusion：The growth inhibitory effect of curcumine on mouse melanom is proved in vivo and in vitro respectively. Curcumine can induce some ceils to apoptosis which may be relevant to downregulation of bcl-2 expression and upregulation of p53 expression as well as exhaustion of GSH in tumor organization.
出处
《中药材》
CAS
CSCD
北大核心
2008年第11期1685-1689,共5页
Journal of Chinese Medicinal Materials
