摘要
采用鱼体外周血细胞微核技术研究新乡市城市污水对泥鳅的遗传毒性,实验各组(A组、B组、C组、D组)中微核率明显高于对照组(P<0.01)。以黄嘌呤氧化酶法检测泥鳅肝组织中的超氧化物歧化酶(SOD)酶活力变化,以赖氏法检测肝组织和血清中的谷草转氨酶(GOT)和谷丙转氨酶(GPT)酶活力变化,研究该污水对泥鳅的生理毒性。结果表明,实验各组肝组织中SOD、GOT和GPT酶活力均低于对照组,且随城市污水浓度升高而减小;血清中的SOD、GOT和GPT酶活力均高于对照组,且随城市污水浓度升高而增强。由此可知,直接排放的城市污水对泥鳅有显著的遗传毒性与生理毒性,水样中有致突变酶活力物质存在。
The micronuclear technique was performed on the fish＇s peripheral blood cells to study the genotoxicity of Xinxiang （Henan, China） domestic wastewater on loach. The micronuclear rates of fishes of the wastewater group （1 week exposure to 100.0%, 50.0%, 33.3% and 25.0% of the wastewater） were significantly higher （P〈 0.01） than that of the control group （0% wastewater）. Using xanthine oxidase enzyme assay, the superoxide dismutase （SOD） activity in liver and serum were measured for the samples prepared from fishes of the two groups. The activity changes of glutamic-oxalacetic transaminase （GOT） and alanine aminotransferase （GPT） in liver and serum were also measured for the same samples to study the physiological toxicity of the domestic wastewater on Loach. The activities of SOD, GOT and GPT in the liver samples of the wastewater group fishes were lower than those of the control group （P〈0.05）, while they were higher in the serum samples of the wastewater group relative to the control; the observed changes were dependent on the wastewater present in the test systems. Such results suggest that discharge of raw domestic wastewater will produce significant genotoxicity and physiological toxicity on loach due to the presence of mutagenic substances in the wastewater.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2008年第11期31-34,共4页
Environmental Pollution & Control
基金
河南省杰出青年基金资助项目(No.0512001800)
关键词
微核
毒性
GOT
GPT
SOD
micronuclear
toxicity
glutamic-oxalacetic transaminase
alanine aminotransferase
superoxide dismutase
