摘要
为了观察脑死亡后不同液体容量治疗对血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)等炎症介质水平改变的影响,选用巴马小型猪15只,随机分为3组各5只,即对照组(C组)、4%改良琥珀酰明胶组(MFG组)及乳酸林格氏液组(RL组)。C组不建立脑死亡模型,给与乳酸林格氏液30ml.kg-1.d-1～40ml.kg-1.d-1。MGF组和RL组为脑死亡组,采用颅内加压法建立脑死亡模型,MFG组给与4%改良琥珀酰明胶溶液30ml.kg-1.d-1,RL组给与乳酸林格氏液30～40ml.kg-1.d-1。分别于麻醉后及首次判定脑死亡后3、6、12、18和24h留取血样,检测血清中TNF-α、IL-1β、IL-6水平。结果发现MGF组较RL组血压和心率平稳;RL组TNF-α、IL-1β和IL-6水平自脑死亡后3h点开始逐渐升高;MFG组TNF-α、IL-1β和IL-6水平脑死亡后3h点开始亦升高,但升高幅度均较RL组低;三组间自脑死亡后3h点开始,组间比较差异有统计学意义。提示脑死亡后血清炎症介质TNF-α、IL-1β和IL-6释放增加,琥珀酰明胶可抑制脑死亡后TNF-α、IL-6、IL-1β等炎症介质的释放,减轻炎症反应,改善机体状况。
The purpose of this study is to investigate the Changes of TNF--α（Tumor necrosis factor--α）,IL--1β（Interleukin--1β） ,IL--6 （Interleukine--6） after the initial brain death of Ba--Ma mini pigs and to investigate the effects of 4% modified fluid gelatin （MFG）on the Changes of inflammatory factors of BA- Ma mini pigs with brain--dead state. 15 Ba --Ma mini pigs were randomly divided into 3 groups： 4% modified fluid gelatin group （group MFG, n= 5）, lactated Ringer＇s solution group （group RL, n=5） and control group （group C, n=5）. In group MFG and group RL the brain--dead model were established by increasing intracranial pressure in a slow and intermittent way, and maintained brain--dead state for 24h by respiration and circulation support. MFG （30 ml · kg^-1 ·d^-1 ） （group MFG） or lactated Ringer＇s solution（30-40 ml · kg^-1 ·d^-1）（group RL）were infused, group C was maintained anesthesia for 24 h but not created brain--dead model,and lactated Ringer＇s solution（30~40 ml · kg^-1 ·d^-1 ） were infused. At time after anesthesia and 3, 6, 12, 18 and 24h after the initial brain death, serum TNF--α, IL--1β, and IL--6 were determined. Group MFG hemodynamic stability than group RL, since 3h after the initial brain death, these parameters for group MFG and group RL began to increase. TNF--α,IL--1β and IL--6 at 3, 6, 12, 18, and 24h after the initial brain death, had significant differences; and compared with group MFG, these parameters for group RL were significantly higher at each time point. The release of TNF--α, IL--1β and IL--6 can be enhanced by the brain death. MFG can diminishing the release of TNF--α, IL--1βand IL--6.
出处
《医学与哲学（B）》
2008年第11期62-64,66,共4页
Medicine & Philosophy(B)
基金
河南省杰出人才创新基金资助项目
项目编号:0421002500
关键词
脑死亡
巴马小型猪
炎症介质
琥珀酰明胶
容量治疗
brain death, mini pigs, inflammatory factors, modified fluid gelatin, volume replacement
