摘要
目的了解变异链球菌、血链球菌和嗜酸乳杆菌的代谢谱图,探讨基于核磁共振的代谢组学方法在口腔致龋菌快速鉴定中的应用。方法分别在改良TPY液体培养基中接种相同密度的变异链球菌、血链球菌及嗜酸乳杆菌菌悬液,采用比浊法计算细菌数,绘制生长曲线。选择这3种细菌生长稳定期的培养液检测核磁共振波谱,用主成分分析法进行数据分析。结果主成分分析显示3组数据内部有集中的聚类关系,可以区分这3种细菌。结论代谢组学分析是一种有良好发展前景的口腔细菌的快速鉴定方法。
Objective To establish the spectra of metabolites that coiled be employed in identification of oral pathogenic bacteria, and try to find a convenient and rapid way to discriminate oral microorganisms Methods Suspensions of Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556 and Loctobocillus ocidophilus ATCC 4356 with same density were preparecd and cultured respectively at improved TPY liquid culture medium. The growth quantity were measured periodically by a turbidimeter. And the growth curves of the inoculated bacteria were completed. The culture solutions in stationary phase of the three bacteria were tested with ^1H-nuclear magnetic resonance （^1H-NMR） spectroscopy respectively. The data of ^1H-NMR spectroscopy results were analyzed by principal components analysis （PCA）. Results The PCA showed the obvious clustering phenomena and the points of three group differentially centralized to three clusters. Therefore, the NMR-based metabonomics profiles could discriminate the three different kinds of bacteria. Conclusion The metabonomics is a promising new technology for developing to a rapid discrimination method of oral pathogenic bacteria.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2008年第5期537-540,共4页
West China Journal of Stomatology
基金
教育部科学技术研究重大项目资助项目(307022)
四川省应用基础研究资助项目(07JY029-072)
关键词
代谢组学
核磁共振
细菌
鉴定
metabonomics
nuclear magnetic resonance
bacterium
identification
