摘要
目的探讨长程体外反搏对高胆固醇血症猪血清中高级氧化蛋白产物（AOPP）和高敏C反应蛋白（hs-CRP）的影响。方法18头雄性乳猪随机分为正常饲养组（n=6）、高脂饲养组（n=6）及高脂饲养＋体外反搏组（n=6）。后2组通过高脂饲养复制高胆固醇血症猪模型并对高脂饲养＋体外反搏组进行36d共36h的长程增强型体外反搏。分别于分组饲养前、反搏前、反搏中期和反搏结束时留取3组动物静脉血，采用分光光度法检测血清AOPP浓度，采用乳胶凝集反应法检测血清hs-CRP浓度。结果高脂饲养组和高脂饲养＋体外反搏组经高脂饲养后血清总胆固醇和低密度脂蛋白明显升高（P〈0．05）。血清AOPP和hs-CRP浓度在分组饲养前3组差异无统计学意义（P〉0．05）。反搏前、反搏中期和反搏结束时，高脂饲养组与高脂饲养＋体外反搏组血清AOPP和hs—CRP浓度较正常饲养组同时期均有显著增高（P〈0．05）；而反搏中期和反搏结束时高脂饲养＋体外反搏组血清AOPP浓度较高脂饲养组显著降低[（95．38±12．66）μmlol／L比（128．46±12．55）μmol／L；（85．78±10．33）μmol／L比（158．22±16．32）μmol／L，P〈0．05]；且反搏中期和反搏结束时高脂饲养＋体外反搏组血清hs-CRP浓度较高脂饲养组也有显著降低[（0．47±0．14）mg／L比（0．62±0．32）mg／L；（0．47±0．16）mg／L比（0．59±0．43）mg／L，P〈0．05]。结论AOPP和hs-CRP参与了高胆固醇血症猪的发病过程。长程体外反搏可能通过减轻机体体内氧化应激和微炎性反应过程，从而阻止高胆固醇血症的病理生理进程。
Objective To explore the effect of long-term enhanced external counterpulsation （EECP） on serum concentrations of advanced oxidation protein products （AOPP） and high-sensitivity C- reactive protein （hs-CRP） in hypercholesterolemic pigs. Methods Eighteen male infant pigs were randomly divided into 3 groups according to their diet：the normal control group （n = 6）, the hypercholesterolemic group （n =6） and the hypercholesterolemie ＋ EECP group （n = 6 ）. While porcine hypereholesterolemic model was built, EECP was performed on animals of the hypercholesterolemic ＋ EECP group, 2 hours every other day lasting for 36 days. Blood sanlples were harvested from all animals to detect the levels of AOPP and hs-CRP before initiating different diet, prior to EECP, during EECP and after completion of EECP respectively. Results The serum concentrations of AOPP and hs-CRP, as well as the levels of serum eholesterone and low density lipoprotein, of both the hypercholesterolemic group and the hypereholesterolemic ＋ EECP group were significantly higher than those of normal control group at these time points： prior to EECP, during EECP and after completion of EECP （ P 〈 0.05 ） , while no difference was observed among three groups before initiating different diet （ P 〉 0.05 ）. However, the AOPP levels of the hypercholesterolemic ＋ EECP group were significantly lower compared with those of hypercholesterolemic group during EECP and after completion of EECP respectively[ （95.38 ±12.66）μmol/L vs （ 128.46± 12. 55 ） μmol/L, （ 85.78 ± 10.33）μmol/L vs （ 158.22 ±16.32）μmol/L, P 〈 0.05 ]. Samely, the hs-CRP concentrations of the hypercholesterolemic ＋ EECP group were also obviously lower compared with those of hypercholesterolemic group during EECP and after completion of EECP respectively [ （0.47 _＋ 0.14） mg/L vs （0.62 ＋ 0.32） rag/L, （0.47 ＋ 0.16） mg/L vs （0.59 ＋ 0.43 ） rag/L, P 〈 0.05 ]. Conclusions AOPP and hs-CRP play an important role in the pathogenesis of hypercbolesterolemia and atherosclerosis. Long-term EECP eliminates the impact of both inflammation and oxidative stress damage from hypercholesterolemia, contributing to postponing initiation and progression of the early atherosclerosis.
出处
《中华生物医学工程杂志》
CAS
2008年第3期200-203,共4页
Chinese Journal of Biomedical Engineering
基金
国家“十五”科技攻关计划基金（2001BA706807）
广东省高等教育厅产学研项目基金（8000-3211501）
关键词
增强型体外反搏
高胆固醇血症
高级氧化蛋白产物
氧化应激
高敏C反应蛋白
Enhanced external counterpulsation
Hypercholesterolemia
Advanced oxidationprotein products
Oxidative stress
High-sensitivity C-reactive protein
