摘要
目的:建立HPLC-MS-MS方法定量测定人血浆中多潘立酮浓度。方法:以地西泮为内标,采用甲醇:0.5%乙酸=80:20为流动相,以Inerstil C18(2.1mm×100mm,3.0μm)色谱柱为分析柱,通过电喷雾离子源(ESI),以正离子多反应监测(MRM)方式进行检测。多潘立酮和地西泮用于定量分析的离子对分别为[M+H]+m/z426.2→m/z175.2和[M+H]+m/z285.0→m/z193.0。结果:多潘立酮线性范围为1～80μg·L-1,定量下限为1μg·L-1(n=6)。日内、日间的RSD均<15%,平均回收率>75%。结论:本方法专属性强,样品处理方便,灵敏度高,适用于多潘立酮临床药动学研究。
Objective：To establish a HPLC-MS-MS method for the determination of domperidone in human plasma.Methods：Diazepam was used as internal standard.Domperidone was separated on an Inerstil C18 column（2.1 mm×100 mm,3.0 μm）.The mobile phase consisted of methanol -0.5% acetic acid（80 ：20）.Electrospray ionization（ESI） was applied and operated in the positive multiple reaction monitoring（MRM） mode,used the transitions of [M ＋ H]^ ＋ m/z 426.2 → m/z 175.2 and [M ＋ H]^＋ m/z 285.0 → m/z 193.0 to quantify domperidone and the internal standard respectively.Results：The linear calibration curve was obtained over the concentration range of 1 ～ 80 μg · L ^-1.The limit of quantification was 1 μg · L ^-1（n = 6）.The inter and intra-day precision（RSD） were less than 15%.The average recovery was above 75%.Conclusion：The method is proved to be specific,convenient,sensitive and suitable for the clinical investigation of domperidone pharmacokinetics.
出处
《中国药物应用与监测》
CAS
2008年第5期19-21,共3页
Chinese Journal of Drug Application and Monitoring
