摘要
目的:探讨玻璃化冷冻未成熟卵母细胞的有效性。方法:根据有无颗粒细胞将实施玻璃化冷冻的GV期卵母细胞分为含颗粒细胞(非裸卵)组和不含颗粒细胞(裸卵)组;将部分GV期卵母细胞体外培养至MⅡ期卵母细胞实施玻璃化冷冻,比较非冷冻IVM组与MⅡ卵玻璃化冷冻组间、裸卵组与非裸卵组间的存活率、成熟率、受精率、卵裂率及囊胚形成率。结果:非裸卵组的成熟率大于裸卵组(P<0.05),而存活率、受精率、2-细胞形成率、>2-细胞形成率之间均无统计学差异(P>0.05)。另外,非冷冻IVM组与GV玻化组间成熟率、受精率、卵裂率均存在显著性差异(P<0.05);非冷冻IVM组与MⅡ期卵玻化组间成熟率、受精率、卵裂率间均存在统计学差异(P<0.05);GV玻化组与MⅡ玻化组间存活率、成熟率、受精率、卵裂率间均无统计学差异(P>0.05)。结论:玻璃化冷冻未成熟卵母细胞需要保留颗粒细胞,同时初步构建了人GV期卵的玻璃化冷冻联合IVM技术的雏形。
Objective： To investigate the efficiency of vitrification of immature oocytes to expand the field of gamete cryopreservation. Methods： The surplus GV oocytes from our ICSI programme were assigned to group with granule cells and group without granule cells. In addition, oocytes from IVM were as control group, GV vitrification group and MⅡ vitrification group as test groups. The survival rate, fertilization rate, cleavage rate and formed blastocyst rate were compared. Results： There was a significant difference of maturation rate between groups with granule cells and without granule cells （P〈0.05）. The survival rate, fertilization rate, cleavage rate （including 2- cell rate and more than 2 cells rate） had no significant difference （P〉0.05）, and the maturation rate, fertilization rate, cleavage rate were significantly different between IVM group and GV vitrification group, as well as IVM group and MⅡ vitrification group （P〈0.05）. But there was no significant difference of survival rate, maturation rate, fertilization rate, cleavage rate between GV vitrification group and MⅡ vitrification group （P〉0.05）. Condusion： 1） Vitrification of human immature oocytes must reserve with granule cells. 2） The platform of human immature oocytes vitrification technology which combine with in vitro maturation technology has already been established.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2008年第10期593-597,共5页
Reproduction and Contraception
基金
国家863计划基金资助项目
项目号:2006AA02Z4A4
