摘要
目的:探讨NK、T混合淋巴细胞(NKTm)与顺铂联合应用于肺巨细胞癌PLA-801D细胞的最佳时序方案,为临床治疗提供实验依据。方法:按加入NK、T混合淋巴细胞(NKTm)和顺铂的时序不同分为7个时序组,每个时序组中设单顺铂组,单NK、T混合淋巴细胞(NKTm)组及联合组,MTT法分别测定各组对PLA-801D细胞的抑制率。结果:各时序组中联合组的抑制率高于单顺铂组和单NK、T混合淋巴细胞(NKTm)组(P<0.01),各时序组联合应用的Q值均大于1.15,且先用NK、T混合淋巴细胞(NKTm)4 h后再用顺铂组的Q值最大(Q=2.297)。结论:体外研究表明,NK、T混合淋巴细胞(NKTm)与顺铂联合应用具有协同效应,先用NK、T混合淋巴细胞(NK-Tm)后4 h再用顺铂为最佳时序方案。
Objective： To investigate the best choice of combined treatment to human pulmoal giant cell carcinoma cells （PLA-801 D）with mixture of NK and T lymphocytes （NKTm cells）and cisplatin （DDP） in different sequences. Methods： The inhibited rates of PLA-801D cells treated by single NKTm cells, single DDP, and combined treatment of NKTm cells and DDP in different sequences were determined with MTr colorimetric assay. Results： The effect of combined treatment of NKTm ceils and DDP excelled the effect of single NKTm cells treatment and single DDP treatment（P 〈 0.01 ）. Values of Q in all the combined group were more than 1.15 ; among all the group, the sequence of using NKTm ceils first, and then 4 h later using DDP had the highest Q value（ Q = 2.297）. Conclusion： Combined treatment of NKTm cells and DDP had synergistic effect to inhibit PLA- 801D cells. The best sequence was to using NKTm cells first, and then 4 h later using DDP.
出处
《军医进修学院学报》
CAS
北大核心
2008年第5期346-348,共3页
Academic Journal of Pla Postgraduate Medical School
关键词
杀伤细胞
天然
T淋巴细胞
顺铂
killer cells, natural
T-Lymphocytes
cisplatin
