摘要
以人参芽胞、二年生人参根、实生苗(茎、叶)为外植体研究了体细胞胚的发生条件,并对其发生过程中可溶性蛋白、相关酶活性及内源激素的变化等进行了研究。结果表明,诱导愈伤组织的培养基为MS+2,4-D4.0mg/L+BA0.2mg/L;在MS+2,4-D1.0mg/L+KT0.2mg/L培养基上继代培养,可获得胚性愈伤组织;在无2,4-D的培养基上可诱导出胚状体。将胚状体转入无任何激素的MS培养基上继续培养,之后转入1/2MS培养基上获得再生植株。在体细胞胚胎发生过程中,可溶性多糖和可溶性淀粉含量在早期胚时较低,可溶性蛋白含量、POD及PPO活性在早期胚时最高;IAA在早期胚时期含量最高,在成熟胚时期ABA含量最高,而ABA/IAA比值在成熟胚时较高,利于体细胞胚的发育成熟。
Rapid propagation of Panax ginseng in tissue culture was reported ; the process of morphogenesis through different path from shoots , stems , leaves and seeds as explants obtained regenerated plant . The callus was induced on MS medium supplemented with 4. 0mg/L 2,4-D and 0.2mg/L BA . The callus was subcuhured on MS medium supplemented with 2.0mg/L 2,4-D and 0.2mg/L KT. Embryogenic callus could be obtained and then lacking 2,4-D we can obtain embryoid. It can grow fast on regenerated on 1/2MS. It is assumed that the origin of somatic MS medium with no growth regulators. Plantlets embryo of Panax ginseng is from the single-cell. The origin of adventitious buds of Panax ginseng is from callus surface or inner. Dissolvability polysaccharide and soluble amylum involve in its embryoid callus and development. High activities of POD and PPO and protein level were necessary to earlier offer material and energy. The contents of IAA is higher in earlier embryo and ABA is higher in mature embryo. The ratios of ABA/IAA are conducive to the development of somatic embryogenesis.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第10期106-112,共7页
China Biotechnology
