摘要
目的:观察脓毒症对小鼠肺相关酶活性的影响,探讨丙酮酸乙脂(Ethyl Pyruvate,EP)在脓毒症中对受损肺脏的作用。方法:采用盲肠结扎穿孔术制备小鼠脓毒症模型,用分光光度法检测肺组织匀浆液中丙二醛(Malonaldehyde,MDA)、丙酮酸(Pyruvate Acid)、乳酸(Lactic Acid)及总抗氧化能力(total anti-oxidative capacity,TAOC)等4种与自由基合成、代谢相关的酶活性,同时采用放射免疫分析测定血清瘦素(leptin)水平。结果:腹腔内注射EP后,肺组织丙二醛、丙酮酸、乳酸及总抗氧化能力等酶活性有不同程度的改变,但结果并非完全抑制或促进。血清leptin水平在EP保护后6h和12h较损伤组各相应时间点显著升高。结论:EP可能通过leptin发挥对脓毒症后受损肺脏的保护作用。
Objective To investigate the protective role of ethyl pyruvate on mouse models of lung injury from sepsis. Methods Mouse sepsis models were established by cecal ligation-perforation. Four enzyme parameters related to synthesis of free radicals in lung homogenized fluids namely malonaldehyde （MDA）, pyruvate acid, lactic acid and total anti-oxidative capacity （TAOC） were determined with spectrophotometry, and serum leptin levels were detected with radioimmunoassay at 3, 6, 9, 12h after operation in these models. Half of the models were treated with intruperitoneal injection of ethyl pyruvate （EP） （75mg/kg）. Results In the models treated with ethyl pyruvate injection, the activity of malonaldehyde, pyruvate acid, lactic acid and total anti-oxidative capacity were affected to certain extent, at some time frames but the results were not unanimously inhibitive or promotive. Serum leptin levels in EP injection models at 6h and 12h after sepsis were significantly higher than those in non-treated models. Conclusion Ethyl pyruvated perhaps exerted its protective effect on sepsis-induced lung injury through increase of leptin levels in the models.
出处
《放射免疫学杂志》
CAS
2008年第5期385-387,共3页
Journal of Radioimmanology
基金
国家自然科学基金面上项目(30670821)
关键词
丙酮酸乙脂
脓毒症
瘦素
丙二醛
ethyl pyruvate, sepsis, leptin, malonaldehyde
