摘要
目的从牛胰脏制备核糖核酸Ⅱ(RNAⅡ),对巴氏灭活法灭活病毒工艺进行验证。方法以苯酚、氯仿抽提法制备,以猪细小病毒(PPV)为指示病毒,观察用巴氏灭活法(60℃,10h)灭活RNAⅡ原液中病毒的效果,用纯度、A260/A280比值、紫外特征峰、完整性、含量变化考察灭活前后制剂变化。结果制备了纯度和活性均较好的RNAⅡ,PPV滴度为7.7logTCID50/0.1mL时灭活液中未检测出PPV,经盲传3代,亦未发现特异性细胞病变,灭活前后制剂无质的变化。结论可以现行方法从牛胰脏制备较高纯度RNAⅡ,巴氏法能对RNAⅡ溶液作有效的病毒灭活处理。
Purpose To purify RNA Ⅱ from calf pancreas and to validate the virus inactivationl by pasteurization. Methods RNA Ⅱ was purified from pancreas by extraction of phenol and chloroform and RNA solution adding the porcine parvovims （PPV）was heated to 60℃ for 10 hours. Purity, A260/A280, special UV absorbanee peak and integrity were matched before and after pasteurization. Results High purity and activity of RNA Ⅱ were purified, and the initial titre of PPV added into RNA Ⅱ solution is 7.71ogTCID50/0.1 mL. The residual virus was undeteetable in the virus inactivation solution and the matched itoms didn＇t change qualitatively. Conclusion The method of purification of RNA Ⅱ and inactivation of virus is effective.
出处
《中国生化药物杂志》
CAS
CSCD
2008年第5期332-334,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
核糖核酸Ⅱ
制备
病毒灭活
巴氏法
RNA Ⅱ
purification
virus inactivation
pasteurization
