摘要
目的采用HPLC法测定咖酚伪麻分散片中的对乙酰氨基酚、盐酸伪麻黄碱和咖啡因。方法采用Diamonsil C18色谱拄（150mm×4．6mm，5μm），流动相为甲醇-0．05mol·L-1磷酸二氢钠溶液（20：80），流速1．0ml·min-1，检测波长210nm。结果对乙酰氨基酚、盐酸伪麻黄碱和咖啡因的线性范围分别为0．0532～1．3355μg（r=0．9999）、0．0489～0．6110μg（r：0．9999）和0．0232～0．2895μg（r=0．9999），平均回收率分别为99．6％、99．7％、99．7％（n=9）。结论所建方法分离效果好、灵敏度高、重复性好、准确可靠。
OBJECTIVE To establish an RP - HPLC method for determination of content of paracetamol, pseudoephedrine hydro- chloride and caffeine in Kafen Weima dispersible tablets. METHODS The samples were seperated by Diamonsil - C18 column （ 150 mm × 4.6 mm ,5 μm） with methanol- 0.05 mol. L-1 NaH2PO4 solution（20 ： 80 ） as mobile phase and detected at 210 nm with flow rate of 1.0 ml. min- 1. RESULTS The calibration curve was linear in the range of 0.0532 - 1. 3355 μg for paracetamol, in the range of 0. 0489 - 0.6110 p,g for pseudoephedrine hydrochloride, in the range of 0. 0232 - 0.2895 μg for caffeine. The average recoveries were 99.6% ,99.7% ,99.7% （ n = 9） , respectively. CONCLUSION The method is good in the aspect of resolution, reproducibility and sensitivity.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第5期606-607,共2页
West China Journal of Pharmaceutical Sciences
