摘要
从土壤中分离到1株高活性自养反硝化菌TD,并对其进行了鉴定和硝酸盐还原特性研究.该菌株为革兰氏阴性短杆菌,严格自养.16SrDNA序列分析表明,该菌株与Thiobacillus denitrificans相似性为99.85%.结合生理生化特性和16SrDNA序列分析,确定菌株TD为脱氮硫杆菌.通过对该菌的生长特性和反硝化特性的研究表明,该菌在初始pH为6.85,32.8℃培养条件下脱氮效果最佳;在初始pH为6.90,29.5℃培养条件下生长最快.该菌生长比较缓慢,没有明显的稳定期,对数生长期阶段的反硝化能力最强,反硝化速率最快,达到了2.245mg·(L·h)-1,在培养过程中培养基pH值明显下降.较高盐度对该菌株的反硝化活性有抑制作用.该菌株的急性毒性实验结果显示,脱氮硫杆菌对健康鱼体几乎无毒,属于无毒性菌株.
A autotrophic denitrifying bacterial strain, TD, was isolated from soil and the strain was identified and characterized. The strain was gram negative, strictly chemolithoautotrophic, and short rod shaped bacterium. The 16S rDNA sequence analysis revealed that strain TD had a similarity of 99.85 % with Thiobacillus denitrificans. According to the morphologic, physiobiochemical characteristics and the analysis of its 16S rDNA, the strain was identified as Thiobacillus denitriftcans. Studies showed that the optimal conditions for denitrification were pH 6.85 and 32.8℃, while the optimal growth conditions were pH 6.90 and 29.5℃ . The bacteria grew slowly with no apparent stable phase. The maximal denitrification rate reached 2. 245 mg·（L·h）^-1, which was found in exponential phase. In the process of the culture, the medium pH decreased significantly. Relatively high salinity restrained the denitrification activity of Thiobacillus denitrificans. The acute toxicity test results showed that ThiobaciUus denitrificans was non-toxic.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2008年第10期2931-2937,共7页
Environmental Science
基金
国家科技支撑计划项目(2006BAD03B06)
上海市重点学科建设项目(Y1101)
关键词
自养反硝化
脱氮硫杆菌
生理生化特性
16S
RDNA
反硝化特性
硝酸盐氮
盐度
毒性
autotrophic denitrification
Thiobacillus denitrificans
biochemical and physiological characters
16S rDNA
denitrification characteristic
nitrate
salinity
toxicity
