摘要
为了探明姬松茸提取物的体外抗病毒感染活性及其作用方式,进而为水产养殖鱼类高效抗淋巴囊肿病毒(lym-phocystis disease virus,LCDV)感染活性物质的开发和鲆蝶类淋巴囊肿病的防治奠定基础,利用热水浸提和酒精沉淀法得到了5种姬松茸提取物组分(E1～5),并利用MTT、细胞病变程度观察等方法研究了5种组分对LCDV感染体外培养大菱鲆鳍细胞(turbot fin cells,TF细胞)的影响作用。细胞毒性实验结果显示,5种组分对体外培养TF细胞均无毒性。细胞病变程度结果表明,本文所得5种姬松茸提取物组分尤其是E5组分具有显著的抗LCDV感染TF细胞的活性。不同方式感染的实验结果进一步显示,5种姬松茸提取物组分抗LCDV感染TF细胞的作用可能主要是通过直接灭活病毒和/或阻断病毒吸附细胞来实现的。
To study the antiviral infective activities of mushroom Agaricus blazei, we extracted its components by hot-water extraction method and alcohol precipitation method. Cytotoxic evaluation indicated that all the five fractions had no cytotoxic activities on cultured turbot fin cells（TF cells）, but all of them showed obvious inhibitory effect on the infective activity of lymphocystis disease virus（LCDV） to TF cells, especially E5. E5 had the highest anti-infective activities. The mechanisms of this function were probably by inactivating virus directly and/or blocking adsorption of viruses to host ceils. These results provide information for the development and utilization of effective antiviral components to LCDV in fish aquaculture.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第5期775-780,840,共7页
Periodical of Ocean University of China
基金
农业部海洋渔业资源可持续利用重点开放实验室开放基金(20060112)
青岛市科技发展计划项目(03-2-HH-1)资助
