摘要
为了解山东小麦种质资源的抗性基因分布规律,利用Pm4、Pm8、Pm13、Pm21等抗白粉病基因的STS、SCAR标记对山东省农科院作物所保存的227份小麦育成品种和442份地方品种进行白粉病抗性基因鉴定。在育成品种中,抗病基因Pm4、Pm8和Pm13都有发现,其中具有Pm4、Pm8和Pm13的材料分别为5份、74份和3份,分别占育成品种的2.2%、32.6%和1.3%。Pm4基因在20世纪90年代开始用于育种,并应用至今;Pm8基因在20世纪70-90年代应用较多;Pm13基因很少应用,Pm21未被应用。在地方品种中,发现具有Pm4的材料17份,占3.8%,未发现Pm8、Pm13和Pm21。Pm4基因在山东地区分布较广,主要分布于德州、潍坊、泰安、淄博等山东省中部地区。此外还发现含有两个抗病基因的聚合材料,如潍麦8号和山农863410含有Pm4+Pm8,烟中1934和鲁农89(4)116含有Pm8+Pm13。
The STS or SCAR markers of the powdery mildew resistance genes of Pm4,Pm8,Pm13 and Pm21 were used to identify the Pm genes in 227 cultivars and 442 landraces in Shandong province.Among 227 cultivars,5,74 and 3 were identified with Pm4,Pm8 and Pm13,accounting for 2.2%,32.6% and 1.3%,respectively.The present study demonstrated that Pm4 had been applied in breeding programs since 1990's and still played important roles in powdery mildew resistance of the current varieties.Pm8 mainly detected in varieties cultivated during the 1970-1990's.So far,Pm13 was not applied widely,whereas Pm21 did not present in Shandong cultivars.Pm4 was widely existed in Shandong landraces.Among 442 landraces,16 were identified with Pm4,accounting for 3.8% of the surveyed landraces.The landraces with Pm4 were mainly collected from the central region of Shandong province,Dezhou,Weifang,Tai-an,Zibo,etc.However,Pm8,Pm13 and Pm21 were not detected in the surveyed landraces.Weimai8 and Shannong863410 were identified with double resistance genes of Pm4 and Pm8,while Yanzhong1934 and Lunong89(4)116 with double resistance genes of Pm8 and Pm13.The detection of these resistant materials to powdery mildew will provide valuable germplasm sources to the breeding program in the future.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2008年第5期905-911,共7页
Journal of Triticeae Crops
基金
山东省农科院创新基金
山东省良种工程项目(2004-3001)
关键词
小麦
白粉病
抗病基因
分子标记
Wheat
Powdery mildew
Resistance genes
Molecular markers