摘要
目的研究阿霉素肾病大鼠肾小管Smad7表达改变与细胞凋亡的关系,并探讨辛伐他汀通过影响Smad7表达和细胞凋亡的肾保护作用机制。方法建立大鼠阿霉素肾病模型,RT-PCR半定量分析肾皮质中Smad7mRNA的表达水平,免疫组化分析Smad7蛋白的定位和表达,用TUNEL法分析细胞凋亡。结果肾病组肾小管中Smad7mRNA、蛋白的表达均上调,与肾小管上皮细胞凋亡呈正相关。辛伐他汀可抑制Smad7表达上调、减少肾小管上皮细胞凋亡。结论阿霉素肾病大鼠肾小管Smad7表达增加与肾小管上皮细胞凋亡相关。辛伐他汀可以通过抑制Smad7表达而降低肾小管上皮细胞凋亡而实现肾保护作用。
Objective To investigate the relationship between the expression of Smad7 and apoptosis in the renal tubular of rats with adriamycin nephropathy and study the renal protect mechanism of simvastatin through effect the expression of Smad7 and apoptosis. Methods Animals were randomly assigned to three groups：normal control, adriamyein nephrosis （ADN）, and ADN treated with simvastatin （10 mg/kg · d）. Expression of Smad7 was evaluated by RT-PCR and immunohistochemistry. Apoptosis was detected by TUNEL method. Results Expression of Smad7 mRNA and protein in ADN rats was significantly upregulated, which showed a positive correlation with tubular cell apoptosis. Simvastatin decreased the level of Smad7. Conclusion The increased expression of Smad7 correlates with tubular cell apoptosis of rats with adriamycin nephropathy. Simvastatin may prevents renal damage through the down-regnlation of Smad7 and the reduction of apoptosis.
出处
《中国实用医药》
2008年第25期10-12,共3页
China Practical Medicine
基金
右江民族医学院科研基金(项目编号:2004042)
