摘要
目的通过建立新西兰白兔肺外科单侧肺缺血-再灌注损伤动物模型，观察其缺血．再灌注损伤特点。方法将96只健康新西兰兔随机分成3组：Ⅰ组为对照组（n=36）；Ⅱ组为单纯阻断肺动脉组（n=30）；Ⅲ组为阻断肺循环组（n=30）。术中监测动物血流动力学指标；分别于开胸时（Ⅰ组）、缺血1小时和再灌注1、2、4、6h（Ⅰ、Ⅱ、Ⅲ组）取肺组织检测MDA；取肺组织作病理学观察，并测定手术侧肺湿／干比。结果各组动物术中血流动力学指标平稳；在缺血1h和再灌注1、2、4、6h，Ⅲ组与Ⅱ组比较，肺湿／干比及肺组织中MDA含量的差异均无统计学意义（P〉0．05），Ⅰ组与Ⅱ、Ⅲ组比较差异有统计学意义（P〈0．05）。在相同的时间点，Ⅱ、Ⅲ组呈现相似的肺损伤病理改变，且较为明显的肺损伤均出现在再灌注4h，再灌注6h肺损伤均呈恢复趋势。结论肺外科中肺动脉阻断和肺循环阻断所致肺缺血-再灌注损伤特点相似，肺外科中肺血管阻断1h是安全的。
Objective To establish pulmonary vessel blocking model in rabbits and to investigate lung ischemia-reperfusion injury in pulmonary surgery. Methods Ninety-six New Zealand rabbits were randomly divided into 3 groups ： group Ⅰ , control group ; group Ⅱ , blocking pulmonary artery ; group Ⅲ, blocking pulmonary arteries and veins. The content of MDA and the wet/dry ratio of the lung tissue were recorded at the time of opening chest （group Ⅰ ）, 1 h ischemia and 1,2,4,6 h reperfusion. Pathological changes of the lung were also observed at each time point. Results Homodynamic parameters were stable in all 3 groups. There was no significant difference in MDA and wet/dry ratio of the lung tissue between group Ⅱ and group Ⅲ （ P 〉 0.05 ） ,but there was significant difference between group Ⅰ and other two groups （P 〈 0.05 ）. Pathological study revealed that similar ischemia-reperfusion injury changes happened in both group Ⅱ and group Ⅲ ,and the obvious injury happened at the time of 4 h reperfusion. At the time of 6 h reperfusion pathological changes in both group Ⅱ and group Ⅲ began to recover. Conclusion Lung ischemia-reperfusion injury caused by blocking pulmonary artery is similar to that caused by blocking pulmonary arteries and veins. It is safe to previously block pulmonary vessels within 1 h during pulmonary surgery.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第9期1116-1118,共3页
Chinese Journal of Experimental Surgery
基金
江苏省社会发展重大项目基金资助项目（BS2006005）
“333高层次人才培养工程”科研资助项目
关键词
肺外科
缺血再灌注损伤
模型
动物
Pulmonary surgery
Ischemia-reperfusion injury
Model,animal
