摘要
以毛竹叶为实材，采用同时水蒸气蒸馏萃取、挥发油提取器、超临界萃取和索氏提取等4种方法提取毛竹叶挥发油，用气质联用仪测试竹叶挥发油，共检出88种挥发性化合物；通过质谱库检索及人工图谱解析，确定了68种化合物结构。采用同时水蒸气蒸馏萃取和挥发油提取器提取的化合物以醇、羧酸、烷烃类为主，C9～C16化合物相对含量分别为69．10％和62．11％；采用超临界萃取提取方法获得的化合物以烷烃、羧酸为主，C9～C16化合物相对含量为22．91％，C17～C25为27．98％，C26～C38为22．78％；采用索氏提取法获得的化合物以烷烃类为主，C26～C38化合物相对含量为58．55％。研究表明，提取方法不同，竹叶挥发油成分在化合物种类、个数及相对含量方面差异很大；采用同时水蒸气蒸馏萃取和挥发油提取器提取技术能够获得较为完整的竹叶挥发油组分信息。
The bamboos leaves collected from Phyllostachys heterocyla cv. pubescens were pretreated with four extraction techniques （ simultaneity distillation extraction （ SDE）, volatile oil distillatory（ VOD）, supercritical fluid extraction（SFE）and Sohlet＇s extraction method （SEM）） to examine volatile oil composition. Capillary GC/MS was used to analyze the profiles of volatile oil from the four extracts of bamboos leaves. 68 components from all of 88 compounds detected in the volatile oil of bamboos leaves were confirmed according to NIST62 library data and manual resolving. It was seen that most of volatile oil composition using two extraction techniques SDE and VOD was alcohols, carboxylic acids, alkane hydrocarbon. The relative ratios of C9 - C16 compounds to total volatile oil were 69.10% and 62.11%, respectively. SFE technique was found most of the volatile oil compounds mainly were alkane hydrocarbons, carboxylic acids. The relative ratio of C9 - C16 was 22.91%, C17 - C25 was 27.98% and C26 - C38 was 22.78%. SEM technique showed that volatile oil compounds mainly were alkane hydrocarbons. The relative ratio of C26 - C38 was 58.55%. It is clear that differences in the profiles of the volatile oil of bamboos leaves exist due to the application of different extraction techniques. The present study indicates that the use of the SDE or VOD to extract bamboos leaves would give more information on volatile oil composition than the other techniques.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2008年第4期135-140,共6页
Journal of Beijing Forestry University
基金
“十一五”国家科技支撑计划课题(2006BAD19B08)
关键词
毛竹叶
提取方法
挥发油成分
Phyllostachys heterocyla cv. pubescens leaves
extraction techniques
volatile oil composition
