摘要
从杭州养鱼塘水样及底泥样品中富集分离得到3株紫色非硫光合细菌,分别命名为HZ-3,HZ-4,HZ-5。通过比较这3株菌对鱼、虾养殖水体的净化效果,筛选出菌株HZ-5净化能力较强,经过5天的处理,该菌株使养鱼塘水样的COD降低24.87%;使养虾池塘水样的COD降低36.99%;使养鱼塘水样的亚硝态氮的降解率达到97.79%。对菌株HZ-5进行了形态学观察、生理生化鉴定、活细胞吸收光谱以及16S rDNA序列分析。16S rDNA序列分析结果表明该菌株与沼泽红假单胞菌的16SrDNA序列有高达99%的同源性,结合形态特征和生理生化特性以及活细胞吸收光谱特征等,将其鉴定为沼泽红假单胞菌(Rhodopseudomonas palustris)。
Three purple non-sulfur bacterial strains were enriched and isolated from the fish pond water and sediments in Hangzhou, they were named HZ-3, HZ-4 and HZ-5 respectively. Among the three purple nonsulfer photosynthetic bacterias, the strain HZ-5 can purify the aquaculture water from fish pond and shrimp pond more efficiently. After 5 days treatment by the strain HZ-5, the COD of fish pond water was decreased by 24.87% ; the COD of shrimp pond water was decreased by 36. 99%; the Nitrite-Nitrogen of fish pond water was decomposed by 97. 79%. The strain HZ-5 was identified as Rhodopseudomonas palustris according to its morphological characteristics, absorption spectrum scanning of live cell and physiological and biochemical properties, and the sequence analysis of 16S rDNA.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第8期91-95,共5页
China Biotechnology
关键词
养殖水体净化
紫色非硫光合细菌
筛选与鉴定
Purification of aquaculture water Purple nonsulfur photosynthetic bacteria Sreening and identification
