摘要
目的:研究β-连环蛋白(β-cat)、细胞周期蛋白D1(cyclin D1)和c-myc基因(c-myc)在正常宫颈组织、宫颈上皮内瘤变(CIN)及宫颈浸润性鳞癌中的表达是否与宫颈癌发生及发展有关。方法:采用PV-9000二步法免疫组化检测试剂,检测正常宫颈组织、CIN和宫颈浸润性鳞癌组织中β-cat、cyclin D1和c-myc的表达。结果:β-cat在正常宫颈组织胞膜中的表达呈强阳性,而在CIN和宫颈浸润性鳞癌胞膜中的表达明显减低,表现为胞质或胞核的异常表达增强,同时cyclin D1和c-myc在CIN和宫颈浸润性鳞癌中表达增强,且与CIN和宫颈浸润性鳞癌发生发展有关。三者在正常宫颈组织、CIN和宫颈浸润性鳞癌中的表达率[分别为4%(2/50)、40%(20/50)、76%(38/50);2%(1/50)、32%(16/50)、58%(29/50)和8%(4/50)、40%(20/50)、68%(34/50)]差异有统计学意义(P均<0.01)。β-cat胞质胞核的异常表达与cyclin D1和c-myc的高表达在CIN和宫颈浸润性鳞癌中均呈显著正相关(相关系数r=0.999)。结论:β-cat的异常表达参与宫颈浸润性鳞癌的发生,并有可能通过激活cyclin D1和c-myc引起细胞增殖和分化失控,促使肿瘤发生、发展。在宫颈癌的发生、发展机制中可能起重要作用。三者同时检查可以作为预测宫颈癌的发生、发展的分子生物学指标。
Objective：To study of the abnormal expressions of β-cat,cyclin D1 and c-myc and its significance in carcinogenesis and progression of human cervical invasive squamous carcinoma.Methods：Semi-quantitative immuno-cyto-chemistry was used to examine expressions of β-cat,cyclin D1 and c-myc in 50 cases of cervical intraepithelial neoplasia and 50 cases of squamous carcinomas.50 cases of normal cervical tissues were served as controls.Result：The expression rates of β-cat,cyclin D1 and c-myc in the normal cervical tissues,cervical intraepithelial neoplasia and squamous carcinomas were significantly different respectively.The abnormal expression rates of β-cat and the high expression rates of cyclin D1 and c-myc had a parallel relationship with the cervical intraepithelial neoplasia and squamous carcinomas,which correlated to the extent of differentiation（r=0.999）.Positive frequency of expression of β-cat was seen in the normal cervical tissues,and it was low in the cervical intraepithelial neoplasia and squamous carcinomas.Positive frequency of expression of cyclin D1 and c-myc were 2%（1/50）,32%（16/50）,58%（29/50）,8%（4/50）,40%（20/50） 68%（34/50） respectively in the normal cervical tissue cervical intraepithelial neoplasia and squamous carcinomas with significant differences（P〈0.01）.Conclusion：The abnormal expressions of β-cat activite and result in uncontrolled proliferation and differentiation,which may play an important role in the carcinogenesis and progression of human cervical invasive squamous carcinomas.The abnormal expressions of β-cat,cyclin D1 and c-myc may provide an index in molecular biology to determine cervical invasive squamous carcinoma and a way of the therapeutic mechanisms of cervical invasive squamous carcinomas.
出处
《新疆医科大学学报》
CAS
2008年第6期641-644,共4页
Journal of Xinjiang Medical University
