摘要
目的探讨非酒精性脂肪性肝病合并胆结石患者外周血单个核细胞代谢相关基因的表达情况。方法选择体重指数（BMI）≥27的肥胖人群，分为三组，单纯肥胖组（n=10），非酒精性脂肪性肝病组（NAFLD组，n=7）和NAFLD伴胆结石组（n=16），另设两组对照，BMI〈25的正常人（n=10）和单纯胆结石组（n=16）。检测比较各组调节脂肪酸合成转录因子甾醇调节因子结合蛋白1C（SREBP-1C）、调节脂肪酸氧化的过氧化酶体增殖物激活受体α（PPARα）和瘦素长型受体（LeptinRb）在外周血单个核细胞的表达。结果三种基因PPARα、SREBP-1c和LeptinRb的mRNA在五组人群的外周血单个核细胞中均有不同程度表达，其中Leptin Rb的表达在单纯肥胖组和NAFI．D伴胆结石组、单纯胆结石组和NAFLD伴胆结石组、NAFLD组和NAFLD伴胆结石组、单纯肥胖组和正常对照组间差异有统计学意义（P值分别-0．037、0．050、0．044和0．038）。NAFLD伴胆结石组LeptinRb的mRNA表达最低。结论NAFLD合并胆结石患者外周血单个核细胞LeptinRbmRNA呈低表达；胆结石可能增加胰岛素抵抗的程度，促进NAFLD进展。
Objective To study the expression of metabolism related genes in peripheral blood mononuclear cells in patients of non alcoholic fatty liver diseases （NAFLD）with cholelithiasis. Methods Patients who had BMI≥27 were divided into obese（n=10）,the NAFLD（n=7） and NAFLD with cholelithiasis （n=16） groups. The subjects who had BM1〈25 （n= 10）and cholelithiasis （n= 16） were served as controls. The expression of peroxisome proliferator-activated receptors （PPAR）α, sterol regulatory element-binding protein（SREBP） lc and Leptin Rb in peripheral blood mononuclear cells were measured. Results The expressions of PPARα,SREBP lc and Leptin Rb in peripheral blood mononuclear cells were different in five groups. The significant difference was found in expression of leptin Rb between NAFLD with cholelithiasis group and the obese group, NAFLD with cholelithiasis group and the cholelithiasis group, NAFI.D with cholelithiasis group and NAFLD group, the obese group and controls （P valiaes=0. 037,0. 050,0. 044and 0. 038, respectively）. The leptin Rb mRNA expression in NAFLD with choletethiasis group was the lowest. Conclusions The low expression of leptin Rb mRNA was found in NAFLD patients with cholelithiasis. Cholelithiasis may increase the extent of insulin resistance and cause NAFLD progression.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2008年第7期488-491,共4页
Chinese Journal of Digestion
关键词
脂肪肝
胆结石
代谢
基因
Fatty liver
Gallstones
Metabolism
Genes
