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不同凝固酶基因型牛源金黄色葡萄球菌主要致病因子的检测及分析 被引量:3

Virulence factors of Staphylococcus aureus of different coagulase genotype
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摘要 采用PCR的方法对不同凝固酶基因型的30株奶牛乳房炎金黄色葡萄球菌的主要致病因子进行检测,包括α溶血素(α-hemolysin)、β溶血素(β-hemolysin)、白细胞毒素F(LukF)、白细胞毒素S(LukS),结果显示:在28株分离株中检测到LukF和LukS;在8株和3株分离株中检测到β、和α溶血素,其分别占分离株的93%、26.7%和10%。这些致病因子在各基因型菌株间的分布并不表现出相关性,而是广泛的分布在各个基因型菌株间。 In this study, PCR method was used to amplify the α-hemolysin, β-hemolysin, LukF and LukS genes from 30 Staphylococcus aureus strains of five different coagulase genetypes isolated from mastitic milk samples. The result showed that 93 % isolates (28) carried LukF and LukS genes and 26.7 % (8) and 10 % (3) isolates have β-hemolysin and α-hemolysin genes, indicating that virulence genes are extensively distributed among the five genetypes.
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2008年第8期608-611,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家“十五”重大专项科技攻关项目(2002BA518A04) 黑龙江省普通高等学校骨干教师创新能力资助计划课题(1055G031)
关键词 奶牛乳房炎 金黄色葡萄球菌 致病因子 凝固酶 bovine mastitis Staphylococcus aureus virulence coagulase
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