摘要
目的:探讨大肠癌组织EMMPRIN和MMP-2蛋白的表达意义.方法:大肠癌患者108例,以正常结、大肠黏膜30例作为对照,应用SABC免疫组化方法检测EMMPRIN和MMP-2蛋白的表达.结果:大肠癌组织EMMPRIN和MMP2蛋白的阳性表达率分别为81.5%(88/108)和86.1%(93/108),明显高于正常大肠黏膜组织(6.7%和20.0%,P<0.01).EMMPRIN和MMP2在中、低分化癌中的强阳性表达率高于高分化癌(P<0.05);浆膜浸润及淋巴结转移组的强阳性率高于无浸润及淋巴结转移组(P<0.01);Dukes C^D期的强阳性表达率高于Dukes A^B期(P<0.01).EMM-PRIN和MMP2的表达一致率为91.4%(85/93),Spearman等级相关性检验分析表明大肠癌组织中EMMPRIN的表达和MMP2的表达之间呈正相关(r=0.608,P<0.01).结论:EMMPRIN和MMP-2与大肠癌的发生、浸润、转移有关,可作为新的大肠癌标记物用于联合检测,具有重要的临床意义.
AIM： To analyze the expressions of EMMPRIN and MMP-2 in human colon cancer, and to evaluate the clinical significance of these two markers in the progression of colon cancer. METHODS： One hundred and eight patients with colon cancer were examined for the expressions of EMMPRIN and MMP-2 by SABC immunohistochemistry, and compared with 30 control cases of normal colon tissue. RESULTS ： The positive expression rates of EMMPRIN and MMP2 in 108 patients with the cancer of colon were 81.5% （88/108） and 86.1% （93/108） respectively, which were significantly higher than those in 30 cases of normal colorectal mucosa（ 6. 67% and 20.0% , P 〈0.01 ）. The expressions of EMMPRIN and MMP2 in moderately-poorly differentiated cancer of colon increased dramatically compared with those in well differentiated tissues （ P 〈 0.05 ） , and the positivity rates of EMMPRIN and MMP2 in tumor tissues with ectoptygma invasion and lymph node metastasis were higher than those without them（ P 〈 0.01 ）. Among Dukes staging, the positivity rates of EMMPRIN and MMP2 in the stages of C-D were higher than those in A-B （ P 〈 0.01 ）. The expression rate of EMMPRIN was positively correlated with the expression of MMP2 in the cancer of colon （ r = 0. 608, P 〈0.01 ）. CONCLUSION： The expressions of EMMPRIN or MMP-2 may be important features of cancer of colon. A combined detection of them may benefit us in prediction of the progression of cancer of colon.
出处
《第四军医大学学报》
北大核心
2008年第14期1319-1321,共3页
Journal of the Fourth Military Medical University
