摘要
目的建立测定尿液样本中表阿霉素的反相高效液相色谱法。方法尿液标本用氯仿-甲醇-异丙醇(5:2:3,V/V/V)重复萃取,萃取液用空气吹干,流动相溶解后用于分析。采用C18柱(150mm×4.6mm,5μm),乙腈-水(35:65,V/V)为流动相,柱温为35.0℃,流速为1.0mL/min,进样量20μL,检测波长为233nm。结果尿液样本中表阿霉素的质量浓度在16～48μg/mL范围内与峰面积呈良好的线性关系,r=0.9977,平均回收率为92.47%。结论该方法比较准确,出峰迅速,专属性强。
Objective To develop the assay method for detecting uric epirubiein concentration by RP- HPLC in order to meet the need of uric epirubiein concentration monitoring and pharmaeokineties. Methods Urine sample was extracted twice with chloroform：methanol： isopropyl alcohol （5 ： 2 ： 3, V/ V/ V）. The C18 analytieal column was used. The measurement wavelength was 233 nm. The mixture of water and aeetonitile （35：65, V/ V） was as the mobile phase at the flow rate of 1.0 mL/min. Results The calibration eurve was linear within the eoneentration range of 16-52 μg/mL. The average reeovery was 92. 47%. Conclusion The method is suitable for the pharmaeokinetie monitor and determination of epirubiein.
出处
《中国药业》
CAS
2008年第15期64-65,共2页
China Pharmaceuticals
