摘要
利用SSR标记研究了32份甜高粱种质的遗传变异,从30对SSR引物中筛选出11对扩增产物是具有稳定多态性的引物。11对引物在供试材料中共扩增出112条带,其中多态性带91条,多态性达81.3%,每对引物扩增出5～16条,平均10.2个,每个位点的多态性信息量(PIC)变化在0.429～0.926之间,平均为0.708。32份甜高粱种质间的遗传相似系数变化范围为0.583～0.969,平均值为0.724,揭示出这32份种质的遗传基础相对比较狭窄。UPGMA聚类分析结果表明,32份供试材料在遗传相似系数0.730处被划分为三大类,HL1和HL2的亲缘关系最近,SART和L-32的亲缘关系最远。
Simple sequence repeats （SSRs） were used to detect genetic diversity among 32 sweet sorghum cultivars. 11 SSR primers selected from 30 primers gave stable profiles amplified in sample of 32 cultivars. 11 primers produced 112 amplified fragments of which 91 （81.3%） were polymorphic. The average number of allele per SSR locus was 10.2 with a range from 5 to 16. The polymorphism information content （PIC） for the SSR loci varied from 0.429 to 0.926 with an average of 0.708. Genetic similarity coefficient among the 32 cultivars ranged from 0.583 to 0.969 with an average of 0.724, it indicated that the genetic relationships were close between them. The UPGMA cluster analysis showed that the 32 cuhivars could be classified into three distinct groups when genetic similarity coefficient was 0.730. The closest relationship was HL1 and HL2, and SART was highly divergent from L-32.
出处
《中国糖料》
2008年第3期40-42,44,共4页
Sugar Crops of China
基金
哈尔滨市科技资助项目(2004AA9CN139)
黑龙江省教育厅资助项目(10541z012)
关键词
甜高粱
SSR
遗传多样性
聚类分析
Sweet sorghum
SSR
Genetic diversity
Cluster analysis
