摘要
为探讨一氧化氮（ＮＯ）在大鼠肝脏缺血再灌注（Ｉ／Ｒ）损伤中的作用，采用大鼠肝脏原位缺血再灌注模型，术前腹腔注射Ｌ精氨酸或Ｌ硝基精氨酸，取肝组织电镜观察，并应用图像立体计量分析技术，分别计算肝细胞线粒体的Ｖｖ、Ｓｖ、ＳＳ、ＮＡ、Ｖ、Ｄ、Ｓ及Ｎｖ８项立体学指标。结果发现，应用Ｌ硝基精氨酸后，肝细胞线粒体肿胀和破坏均较Ｉ／Ｒ组严重，而注射Ｌ精氨酸后，线粒体的８项指标与Ｉ／Ｒ组无差异。提示，ＮＯ在大鼠肝脏缺血再灌注损伤中起保护作用。
To investigate the effects of nitric oxide on the change of hepatacytic ultrastructure during hepatic ischemia/reperfusion in rats,The rat model of in situ hepatic ischemia/reperfusion injury was intraperitoneally injected with Larginine or NGnitroLarginineHepatic tissue samples were randomly observed with a transmission electron microscopeUsing stereologic methods for analysis of electron micrographs,the changes of hepatocytic ultrostructure were studiedEight parameters of stereology:volumetric density(Vv),surface density(Sv),specific surface(SS),numerical density on area(NA),mean diameter(D),mean volume(V),mean area(S),and numerical density(Nv),were calculatedThe results showed that mitochondria were significantly swollen and ruptured in NNA group(administration of NGnitroLarginine)However,the 8 parameters of mitochondria had no difference between Arg group(treatment with Larginine)and I/R groupThese findings demonstrate that NO may play a protective role in hepatic ischemia/reperfusion injury in rats
关键词
一氧化氮
超微结构
肝脏
大鼠
缺血再灌注
nitric oxide
ischemia/reperfusion
ultrostructure
liver rats
