摘要
目的在基因转录水平了解肝再生中肝细胞的生长和分化情况。方法用搜集网站资料和查阅相关论文等方法获得参与肝细胞生长和分化基因,用大鼠基因组230 2.0芯片检测它们在大鼠肝再生（LR）中的表达情况,通过比较手术组和假手术组中基因表达差异性以确定上述基因中的肝再生相关基因。结果初步证实上述基因中110个基因与肝再生相关。肝再生启动（PH后0.5～4h）、G0/G1过渡（PH后4～6h）、细胞增殖（PH后6～66h）、细胞分化和组织结构功能重建（PH后72～168h）等4个阶段起始表达的基因数为63、11、43和3,基因总表达的次数为63、43、101和80,表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共表达上调488次,下调248次,分为6种表达方式,表明肝再生中细胞生理生化活动的多样性和复杂性。结论肝细胞生长和分化贯穿于整个肝再生中。
Objective To investigate the hepatocyte growth and differentiation during rat liver regeneration （LR） at transcriptional level. Methods The related genes were obtained by online data collection and literature review, and the gene expressions in rat regenerating liver were checked by Rat Genome 230 2.0 array. The relativity of the LR-related genes was identified by comparing discrepancies in gene expression changes between partial hepatectomy （PH） group and sham operation （SO） group. Results One hundred and ten genes were found to be related with liver regeneration. The initial and total expressing gene numbers occurring in the initiation phase of liver regeneration （0.5-4 hours after PH）, G0/G1 transition （4-6 hours after PH）, cell proliferation （6-66 hours after PH）, cell differentiation and structure-function reconstruction （72-168 hours after PH） were 63, 11., 43, 3 and 63, 43, 101, 80 respectively, indicating that the related genes were mainly triggered during the initiation phase, and worked at different phases. The total frequencies of their up- and down-regulation expression were 488 and 248, respectively, demonstrating that the expression of the major genes increased, and that of the minority decreased. Their expression profiles were classified into 6 types, displaying that the activities mentioned above were diverse and complicated during liver regeneration. Conclusion Hepatocyte growth and differentiation go through the whole liver regeneration.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第3期290-295,共6页
Acta Anatomica Sinica
基金
国家973计划前期研究专项基金资助项目(2006CB708506)
