摘要
目的初步探讨斑蝥酸钠对人胃癌裸鼠皮下移植瘤生长的作用及其可能的作用机制。方法建立人胃癌细胞系BGC823裸鼠皮下移植瘤模型，观察斑蝥酸钠对移植瘤生长的影响，同时应用免疫组织化学和TUNEL法分别检测斑蝥酸钠作用前后移植瘤细胞的增殖活性和细胞凋亡情况。结果不同浓度的斑蝥酸钠均能明显抑制裸鼠皮下移植瘤的生长（P〈0．01）；斑蝥酸钠作用后肿瘤细胞的增殖活性明显低于作用前（P〈0．01）；斑蝥酸钠作用后肿瘤细胞的凋亡指数显著高于对照组（P〈0．01）。结论斑蝥酸钠对人胃癌裸鼠皮下移植瘤的生长具有明显的抑制作用，其作用机制可能是通过抑制肿瘤细胞增殖或诱导肿瘤细胞凋亡而实现的。
Objective To study the effect of sodium cantharidinate on the growth of human gastric cancer grafted onto nude mice. Methods Nude mice xenograft models of human gastric cancer were established. BGC823 was injected peritoneal and the mice were weighed. The proliferating and apoptosis rates of xenografts was evaluated by TUNEL assay and immunohistochemical staining respectively. Results The xenografts were obviously inhibited with various dosage of sodium cantharidinate（P 〈 0.01 ）, the proliferating rate of tumour cells after using sodium cantharidinate was lower than that before using sodium cantharldinate（ P 〈 0.01 ）, but apoptosis rate of tumour cells after using sodium cantharidinate was higher than that before using sodium cantharidinate（ P 〈 0.01 ）. Conclusion Sodium cantharidinate can inhibit gastric cancer growth by inhibiting tumour cell proliferating or inducing cell apoptosis.
出处
《中国基层医药》
CAS
2008年第4期536-538,共3页
Chinese Journal of Primary Medicine and Pharmacy
基金
安徽省教育厅自然科学研究项目（KJ20078201）
关键词
胃肿瘤
斑蝥素
肿瘤移植
小鼠
裸
Stomach neoplasms
Cantharidin
Neoplasm transplantation
Mice,nude
