摘要
目的葛根是药食同源的植物,为了全面反映葛根对骨代谢的作用,本研究采用了未经任何提取的葛根,观察了其对去卵巢骨质疏松模型小鼠的骨密度和骨组织构造的作用。方法麻醉下切除小鼠双侧卵巢,制作雌激素缺乏所致的骨质疏松模型后,令各组小鼠每天分别摄取含有葛根低、中和高剂量的饲料,并设正常组、模型组和雌二醇组。4周后,对小鼠股骨骨密度、骨微细构造以及子宫的作用进行检测。结果骨密度检测表明,低剂量葛根显著抑制了雌激素缺乏所致股骨骨密度的下降;中剂量葛根完全抑制了这种下降;葛根高剂量组与正常组相比,显著增加了骨密度,其作用强度与雌二醇相当。骨组织形态学分析显示,因雌激素缺乏导致的股骨远端海绵骨的骨量减少、骨小梁宽度的下降以及骨小梁间距的增大,被低剂量葛根显著抑制,被中剂量葛根完全抑制。另外,高剂量葛根显著增加了骨量和骨小梁的宽度。子宫的质量分析显示,小剂量葛根没有明显刺激子宫的作用,中、高剂量葛根轻度抑制了子宫质量的下降,但其强度仅为雌二醇的1/10。葛根抑制骨量减少的作用机制,可能性与抑制破骨细胞的数量有关。结论葛根不同于雌二醇显著刺激子宫,而具有与雌二醇相当的抗骨质疏松作用,有可能成为预防和治疗女性闭经后骨质疏松症安全而有效的食品或药物。
Objective Radix Puerariae （RP）, the root of Puerariae labata （Wind） Ohwi, a wild creeper leguminous plant, is an important crude edible herb and one of the earliest traditional Chinese medicine for various medicinal purposes. The isoflavonoid derivatives extracted from RP have been found to prevent bone loss, but there are not any reports the overall crude herb of RP in bone metabolism. Methods To examine the possible role of RP in bone metabolism, female mice were ovariectomized （OVX）, and administration with low, middle and high dose of RP or 17-beta-estradiol for 4 weeks. Results The whole femoral bone mineral density （BMD） was significantly reduced by OVX, and the decrease in BMD was significantly inhibited by low dose of RP, completely recovered by middle dose of RP, in a dose-dependent manner. Administration of PR, at high dose, significantly increased the whole femoral BMD compared with the sham, and is similar to E2 on OVX mice. Histological analysis of femoral metaphysis shown that administration with middle dose of RP completely prevented the decrease in trabecular bone volume （BV/TV） and trabecular thickness （Tb. Th） and restored the increase in trabecular apace （Tb. Sp） in OVX mice. High dose of PR further increased BV/TV and Tb. Th and reduced Tb. Sp compared with the sham. In OVX mice, the uterine weight declined, intake of low dose of PR did not affect uterine weight, and intake of middle and high dose of PR have a little stimulating in uterine weight but significantly different from E2 in OVX mice. Conclusions These results suggest that PR may represent a potential alternative medicine for hormone replacement therapy （HRT） in the prevention of osteoporosis in postmenopausal women.
出处
《中国骨质疏松杂志》
CAS
CSCD
2008年第5期349-354,共6页
Chinese Journal of Osteoporosis
关键词
葛根
雌二醇
骨密度
骨量
骨质疏松
Radix Puerariae
17-beta-estradiol
Bone mineral density
Bone histomorphometry
Hormone replacement therapy
