摘要
目的研究熊果酸(Ursolic acid,UA)对小鼠T细胞活化和增殖的影响,并探讨其免疫调节机制。方法以刀豆蛋白A(ConA)诱导小鼠T细胞活化和增殖,用MTT法测定细胞增殖,利用流式细胞术检测T细胞早期活化标志CD69、中期活化标志CD25与晚期活化标志CD71的表达情况。结果在ConA刺激下48 h后,小鼠T淋巴细胞MTT吸收度显著高于正常对照组,差异具有统计学意义(P<0.01)。熊果酸(10～40μM)组与ConA组比较显著降低了MTT吸收度,差异具有统计学意义(P<0.01)。在ConA刺激淋巴细胞18 h后,小鼠T细胞CD3+CD69+表达率显著高于正常对照组,差异具有统计学意义(P<0.01),ConA刺激36 h后,T细胞CD3+CD25+表达率显著高于正常对照组,差异具有统计学意义(P<0.01),ConA刺激48 h后,T细胞CD3+CD71+显著高于正常对照组,差异具有统计学意义(P<0.01)。熊果酸组在浓度为10～40μM时与ConA组比较,均能显著降低CD25、CD69、CD71的表达率,差异具有统计学意义(P<0.01)。结论熊果酸可以显著的抑制多克隆刺激剂ConA介导的小鼠T细胞的活化和增殖。
Objective To investigate the effects of ursolic acid on the proliferation and activation of T lymphocytes induced by concanavalin A and the immunoregulatory mechanism. Method The activation and proliferation of T lymphocyte in mice were stimulated by concanavelina （ConA）. The proliferation of the cell was detected with MTT assay, and expression of CD69,CD25,CD71 were detected analyzed with Fluorescence conjugated monoclonal antibodies and flowcytometry. Result Ursolic acid inhibited the expression of CD69, CD25, and CD71 in activated T cells in response to ConA in a concentration-dependent manner. Ursolic acid caused a dose-dependent suppression of T cell proliferation to ConA. Conclution Ursolic acid can significantly inhibit ConA mediated mouse T lymphocyte＇s activation and proliferation.
出处
《湖南中医药大学学报》
CAS
2008年第2期16-18,共3页
Journal of Hunan University of Chinese Medicine
基金
国家自然科学基金资助项目(30371833)
湖南省科学技术厅科技计划(2007SK3030)
