摘要
利用固定有辣根过氧化物酶的毛细管柱作为反应器与流通池，建立了一种简单、快速检测过氧化氢的流动注射化学发光分析新方法。当鲁米诺和过氧化氢的混合溶液流经反应器时，辣根过氧化物酶催化过氧化氢氧化鲁米诺产生化学发光。在选定的实验条件下，化学发光信号与过氧化氢的浓度在3．0×10^-8-1．0×10^-4 mol/L范围内分段呈线性关系，检出限为1×10^-8 mol/L（S／N=3），相对标准偏差1．4％（1．0 μmol/L，n=9）。该方法用于雨水中过氧化氢的测定，结果满意。
A novel flow-injection chemiluminescence（CL） system for the determination of hydrogen peroxide was reported. A capillary column immobilized horseradish peroxidase（HRP） was utilized as a flow CL cell. The CL emission is generated from the reaction between hydrogen peroxide and luminol passing through the column under the catalysis of HRP. The calibration curve for hydrogen perox- ide was linear from 3.0 × 10^-8 to 1.0 x 10^ -4 mol/L and the detection limit was 1 × 10 ^-8 mol/L with a relative standard deviation of 1.4%（ 1.0 μmol/L, n = 9）. The system developed has been applied successfully to the determination of hydrogen peroxide in rainwater samples.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第4期419-422,共4页
Journal of Instrumental Analysis
关键词
流动注射分析
化学发光
毛细管柱
过氧化物酶
过氧化氢
flow injection analysis
chemiluminescence
capillary column
horseradish peroxidase
hydrogen peroxide
